User:Melissa Novy/Notebook/CHEM-571/2012/11/13

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==Entry title==
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==Objectives==
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* Insert content here...
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* Analyze Au-HRP solutions with UV-vis spectroscopy.
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* Analyze solutions of Au-HRP and Au-lysozyme with AAS.
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* Conduct ADA activity assay on ADA fractions using adenosine.
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==ADA Activity Assay==
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* 50 mL of 0.05 M sodium phosphate buffer was made with deionized water.  The pH of the solution was adjusted to 7.4 using 3 drops of 12 M HCl.
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* 5 mL of 0.1 mM adenosine was made.
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** 100 mg of adenosine was dissolved in 1 mL of 0.05 M phosphate buffer, giving a solution of 0.00373 M adenosine.
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** 0.134 mL of 0.00373 M adenosine was diluted to a final volume of 5 mL with 0.05 M phosphate buffer, giving a solution of 0.1 mM adenosine.
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  '''(0.001 g)/(268.02 g/mol) = 3.73×10<sup>-6</sup> mol'''
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  '''(3.73×10<sup>-6</sup> mol)/0.001 L = 0.00373 M'''
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  '''(0.00373 M)(V<sub>1</sub>)=(5 mL)(0.1×10<sup>-3</sup> M)'''
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  '''V<sub>1</sub> = 0.134 mL'''

Revision as of 14:09, 13 November 2012

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Objectives

  • Analyze Au-HRP solutions with UV-vis spectroscopy.
  • Analyze solutions of Au-HRP and Au-lysozyme with AAS.
  • Conduct ADA activity assay on ADA fractions using adenosine.

ADA Activity Assay

  • 50 mL of 0.05 M sodium phosphate buffer was made with deionized water. The pH of the solution was adjusted to 7.4 using 3 drops of 12 M HCl.
  • 5 mL of 0.1 mM adenosine was made.
    • 100 mg of adenosine was dissolved in 1 mL of 0.05 M phosphate buffer, giving a solution of 0.00373 M adenosine.
    • 0.134 mL of 0.00373 M adenosine was diluted to a final volume of 5 mL with 0.05 M phosphate buffer, giving a solution of 0.1 mM adenosine.
  (0.001 g)/(268.02 g/mol) = 3.73×10-6 mol
  (3.73×10-6 mol)/0.001 L = 0.00373 M
  (0.00373 M)(V1)=(5 mL)(0.1×10-3 M)
  V1 = 0.134 mL



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