User:Melissa Novy/Notebook/CHEM-571/2012/11/06 - Revision history

Melissa Novy: /* UV-Vis of Au-Lysozyme Solutions */

Melissa Novy — Tue, 27 Nov 2012 18:54:13 GMT

UV-Vis of Au-Lysozyme Solutions

←Older revision		Revision as of 18:54, 27 November 2012
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	* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.		* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.
	<br>		<br>
-	[[Image:~~AuNP~~-~~Lysozyme_Absorbance~~.~~jpg~~]]	+	[[Image:UV-Vis_Spectra_of_AuLys.JPG]]
	* A peak at around 530 nm was observed for the following Au-lys solutions: 30, 40, 50, and 60. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.		* A peak at around 530 nm was observed for the following Au-lys solutions: 30, 40, 50, and 60. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.
	* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40, 50, and 60 mole ratio Au-lys solutions.		* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40, 50, and 60 mole ratio Au-lys solutions.

Melissa Novy: /* UV-Vis of Au-Lysozyme Solutions */

Melissa Novy — Tue, 13 Nov 2012 19:26:27 GMT

UV-Vis of Au-Lysozyme Solutions

←Older revision		Revision as of 19:26, 13 November 2012
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	<br>		<br>
	[[Image:AuNP-Lysozyme_Absorbance.jpg]]		[[Image:AuNP-Lysozyme_Absorbance.jpg]]
-	* A peak at around 530 nm was observed for the following Au-lys solutions: ~~20,~~ 30, 40, 50, and 60. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.	+	* A peak at around 530 nm was observed for the following Au-lys solutions: 30, 40, 50, and 60. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.
	* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40, 50, and 60 mole ratio Au-lys solutions.		* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40, 50, and 60 mole ratio Au-lys solutions.

Melissa Novy: /* UV-Vis of Au-Lysozyme Solutions */

Melissa Novy — Tue, 13 Nov 2012 19:23:51 GMT

UV-Vis of Au-Lysozyme Solutions

←Older revision		Revision as of 19:23, 13 November 2012
Line 27:		Line 27:
	<br>		<br>
	[[Image:AuNP-Lysozyme_Absorbance.jpg]]		[[Image:AuNP-Lysozyme_Absorbance.jpg]]
-	* A peak at around 530 nm was observed for the following Au-lys solutions: 20, 30, 40, ~~and~~ 50. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.	+	* A peak at around 530 nm was observed for the following Au-lys solutions: 20, 30, 40, 50, and 60. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.
-	* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40 ~~and~~ 50 mole ratio Au-lys solutions.	+	* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40, 50, and 60 mole ratio Au-lys solutions.

Melissa Novy: /* Objectives */

Melissa Novy — Thu, 08 Nov 2012 17:56:00 GMT

Objectives

←Older revision		Revision as of 17:56, 8 November 2012
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	==Objectives==		==Objectives==
	* Obtain ADA protein using HPLC.		* Obtain ADA protein using HPLC.
		+	** Please refer to the [[User:Melissa_Novy/Notebook/CHEM-571/2012/10/03\|protocol]]. No changes were made to the protocol and three fractions of ADA were obtained.
	* Redo cell transformation.		* Redo cell transformation.
	* Analyze Au-lysozyme samples with UV-vis.		* Analyze Au-lysozyme samples with UV-vis.

Melissa Novy: /* UV-Vis of Au-Lysozyme Solutions */

Melissa Novy — Wed, 07 Nov 2012 05:03:46 GMT

UV-Vis of Au-Lysozyme Solutions

←Older revision		Revision as of 05:03, 7 November 2012
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	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06\|Dhea Patel's entry]] for pictures of the solutions.		* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06\|Dhea Patel's entry]] for pictures of the solutions.
	* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.		* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.
-	~~IMAGE~~	+	<br>
-	~~DATA ANALYSIS~~	+	[[Image:AuNP-Lysozyme_Absorbance.jpg]]
-		+	* A peak at around 530 nm was observed for the following Au-lys solutions: 20, 30, 40, and 50. These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04\|past work]] with AuNP formation with BSA.
		+	* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40 and 50 mole ratio Au-lys solutions.


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Melissa Novy: /* Objectives */

Melissa Novy — Tue, 06 Nov 2012 20:44:53 GMT

Objectives

←Older revision		Revision as of 20:44, 6 November 2012
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	* Obtain ADA protein using HPLC.		* Obtain ADA protein using HPLC.
	* Redo cell transformation.		* Redo cell transformation.
-	* Analyze ~~lysozyme~~-~~AuNP~~ samples with UV-vis.	+	* Analyze Au-lysozyme samples with UV-vis.
		+
		+	==UV-Vis of Au-Lysozyme Solutions==
		+	* Au-lysozyme solutions made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/10/31\|2012/10/31]] were obtained.
		+	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06\|Dhea Patel's entry]] for pictures of the solutions.
		+	* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.
		+	IMAGE
		+	DATA ANALYSIS
		+

Melissa Novy: /* Objectives */

Melissa Novy — Tue, 06 Nov 2012 19:57:53 GMT

Objectives

←Older revision		Revision as of 19:57, 6 November 2012
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	==Objectives==		==Objectives==
-	* Obtain ADA protein ~~with~~ HPLC.	+	* Obtain ADA protein using HPLC.
	* Redo cell transformation.		* Redo cell transformation.
	* Analyze lysozyme-AuNP samples with UV-vis.		* Analyze lysozyme-AuNP samples with UV-vis.

Melissa Novy at 19:57, 6 November 2012

Melissa Novy — Tue, 06 Nov 2012 19:57:30 GMT

←Older revision		Revision as of 19:57, 6 November 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	==~~Entry title~~==	+	==Objectives==
-	* ~~Insert content here~~...	+	* Obtain ADA protein with HPLC.
		+	* Redo cell transformation.
		+	* Analyze lysozyme-AuNP samples with UV-vis.

Melissa Novy: Autocreate 2012/11/06 Entry for User:Melissa_Novy/Notebook/CHEM-571

Melissa Novy — Tue, 06 Nov 2012 19:51:34 GMT

Autocreate 2012/11/06 Entry for User:Melissa_Novy/Notebook/CHEM-571

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