User:Melissa Novy/Notebook/CHEM-571/2012/11/06

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Objectives)
(UV-Vis of Au-Lysozyme Solutions)
Line 24: Line 24:
* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06|Dhea Patel's entry]] for pictures of the solutions.
* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/11/06|Dhea Patel's entry]] for pictures of the solutions.
* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers.  It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.
* It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers.  It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.
-
IMAGE
+
<br>
-
DATA ANALYSIS
+
[[Image:AuNP-Lysozyme_Absorbance.jpg]]
-
 
+
* A peak at around 530 nm was observed for the following Au-lys solutions: 20, 30, 40, and 50.  These peaks are likely due to the presence of gold nanoparticles, based on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/04|past work]] with AuNP formation with BSA.
 +
* It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions.  However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40 and 50 mole ratio Au-lys solutions.
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
-
|}
+
 
__NOTOC__
__NOTOC__

Revision as of 01:03, 7 November 2012

Search this Project

Customize your entry pages

Objectives

  • Obtain ADA protein using HPLC.
  • Redo cell transformation.
  • Analyze Au-lysozyme samples with UV-vis.

UV-Vis of Au-Lysozyme Solutions

  • Au-lysozyme solutions made on 2012/10/31 were obtained.
  • Please refer to Dhea Patel's entry for pictures of the solutions.
  • It was observed that the 20 and 30 mole ratio Au-lysozyme solutions did not form fibers, while the rest of the solutions contained dark purple fibers. It appeared that more fibers were present as the mole ratio of Au to lysozyme increased, such that the 130 Au-lysozyme solution contained the most fibers.


Image:AuNP-Lysozyme_Absorbance.jpg

  • A peak at around 530 nm was observed for the following Au-lys solutions: 20, 30, 40, and 50. These peaks are likely due to the presence of gold nanoparticles, based on past work with AuNP formation with BSA.
  • It can be inferred that no gold nanoparticles were suspended in solution for the remaining solutions. However, as it was visually observed that fibers had formed in all solutions except those at 20 and 30 mole ratio Au-lys, this implies that both fibers and gold nanoparticles were present in the 40 and 50 mole ratio Au-lys solutions.



Personal tools