User:Melissa Novy/Notebook/CHEM-571/2012/10/31 - Revision history

Melissa Novy: /* Objectives */

2012-11-07T04:55:07Z

Objectives

←Older revision		Revision as of 04:55, 7 November 2012
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	==Objectives==		==Objectives==
-	* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of ~~lysozyme to~~ Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.	+	* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of Au:lysozyme of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

	==Au-Lysozyme Solutions==		==Au-Lysozyme Solutions==

Melissa Novy: /* Au-Lysozyme Solutions */

2012-11-06T20:40:40Z

Au-Lysozyme Solutions

←Older revision		Revision as of 20:40, 6 November 2012
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	<br>		<br>
	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31\|Dhea Patel's entry]] for calculations, actual molarity, and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.		* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31\|Dhea Patel's entry]] for calculations, actual molarity, and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
		+	* The Au-lysozyme solutions were wrapped in foil to prevent photodegradation and placed in an oven for 4 hours at 80°C.

Melissa Novy: /* Au-Lysozyme Solutions */

2012-11-06T20:39:48Z

Au-Lysozyme Solutions

←Older revision		Revision as of 20:39, 6 November 2012
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	* A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05\|2012/09/05]] was obtained.		* A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05\|2012/09/05]] was obtained.
	* 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.		* 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.
-	** 0.005 g of lysozyme ~~was added~~ to 5 mL of distilled H<sub>2</sub>O.	+	** Add 0.005 g of lysozyme to 5 mL of distilled H<sub>2</sub>O.
-	~~CALCULATION~~	+	'''(70×10<sup>-6</sup> M lysozyme)(0.005 L)=3.5×10<sup>-7</sup> mol lysozyme'''
-	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31\|Dhea Patel's entry]] for calculations and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.	+	'''(3.5×10<sup>-7</sup> mol)(14300 g/mol)=0.005005 g lysozyme'''
		+	<br>
		+	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31\|Dhea Patel's entry]] for calculations, actual molarity, and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.

Melissa Novy at 20:18, 6 November 2012

2012-11-06T20:18:56Z

←Older revision		Revision as of 20:18, 6 November 2012
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	==Objectives==		==Objectives==
	* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.		* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.
		+
		+	==Au-Lysozyme Solutions==
		+	* A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05\|2012/09/05]] was obtained.
		+	* 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.
		+	** 0.005 g of lysozyme was added to 5 mL of distilled H<sub>2</sub>O.
		+	CALCULATION
		+	* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31\|Dhea Patel's entry]] for calculations and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.

Melissa Novy at 19:18, 31 October 2012

2012-10-31T19:18:25Z

←Older revision		Revision as of 19:18, 31 October 2012
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->

-	==~~Entry title~~==	+	==Objectives==
-	* ~~Insert content here..~~.	+	* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

Melissa Novy: Autocreate 2012/10/31 Entry for User:Melissa_Novy/Notebook/CHEM-571

2012-10-31T19:07:28Z

Autocreate 2012/10/31 Entry for User:Melissa_Novy/Notebook/CHEM-571

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