User:Melissa Novy/Notebook/CHEM-571/2012/10/31

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(Objectives)
 
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==Objectives==
==Objectives==
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* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.
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* Make solutions of lysozyme and HAuCl<sub>4</sub> at mole ratios of Au:lysozyme of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.
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==Au-Lysozyme Solutions==
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* A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05|2012/09/05]] was obtained.
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* 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.
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** Add 0.005 g of lysozyme to 5 mL of distilled H<sub>2</sub>O.
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  '''(70×10<sup>-6</sup> M lysozyme)(0.005 L)=3.5×10<sup>-7</sup> mol lysozyme'''
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  '''(3.5×10<sup>-7</sup> mol)(14300 g/mol)=0.005005 g lysozyme'''
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<br>
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* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Dhea Patel's entry]] for calculations, actual molarity, and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
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* The Au-lysozyme solutions were wrapped in foil to prevent photodegradation and placed in an oven for 4 hours at 80°C.

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Objectives

  • Make solutions of lysozyme and HAuCl4 at mole ratios of Au:lysozyme of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

Au-Lysozyme Solutions

  • A 10501 μM solution of HAuCl4 in distilled H2O, made on 2012/09/05 was obtained.
  • 5 mL of 70 μM solution of lysozyme in distilled H2O was made.
    • Add 0.005 g of lysozyme to 5 mL of distilled H2O.
  (70×10-6 M lysozyme)(0.005 L)=3.5×10-7 mol lysozyme
  (3.5×10-7 mol)(14300 g/mol)=0.005005 g lysozyme


  • Please refer to Dhea Patel's entry for calculations, actual molarity, and exact volumes of water, HAuCl4, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
  • The Au-lysozyme solutions were wrapped in foil to prevent photodegradation and placed in an oven for 4 hours at 80°C.



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