User:Melissa Novy/Notebook/CHEM-571/2012/10/31

(Difference between revisions)

Revision as of 16:39, 6 November 2012

Make solutions of lysozyme and HAuCl₄ at mole ratios of lysozyme to Au of 20, 30, 40, 50, 60, 70, 80, 100, 120, 130.

A 10501 μM solution of HAuCl₄ in distilled H₂O, made on 2012/09/05 was obtained.
5 mL of 70 μM solution of lysozyme in distilled H₂O was made.
- Add 0.005 g of lysozyme to 5 mL of distilled H₂O.

  (70×10^-6 M lysozyme)(0.005 L)=3.5×10^-7 mol lysozyme
  (3.5×10^-7 mol)(14300 g/mol)=0.005005 g lysozyme

Please refer to Dhea Patel's entry for calculations, actual molarity, and exact volumes of water, HAuCl₄, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.

@@ Line 21: / Line 21: @@
 * A 10501 μM solution of HAuCl<sub>4</sub> in distilled H<sub>2</sub>O, made on [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/05|2012/09/05]] was obtained.
 * 5 mL of 70 μM solution of lysozyme in distilled H<sub>2</sub>O was made.
-** 0.005 g of lysozyme was added to 5 mL of distilled H<sub>2</sub>O.
+** Add 0.005 g of lysozyme to 5 mL of distilled H<sub>2</sub>O.
-CALCULATION
+   '''(70×10<sup>-6</sup> M lysozyme)(0.005 L)=3.5×10<sup>-7</sup> mol lysozyme'''
-* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Dhea Patel's entry]] for calculations and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.
+   '''(3.5×10<sup>-7</sup> mol)(14300 g/mol)=0.005005 g lysozyme'''
+<br>
+* Please refer to [[User:Dhea_Patel/Notebook/Experimental_Biological_Chemistry_Notebook/2012/10/31|Dhea Patel's entry]] for calculations, actual molarity, and exact volumes of water, HAuCl<sub>4</sub>, and lysozyme solutions added to each test tube to make the Au-lysozyme solutions.