User:Melissa Novy/Notebook/CHEM-571/2012/09/19/2012/09/25: Difference between revisions

Revision as of 10:18, 25 September 2012

Project name

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Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.

Please refer to entry on 2012/09/19 for luminol stock solution protocol and calculations.
Actual mass luminol: 0.00443 g
Actual concentration luminol: 2.5 mM
Actual mass Na₂CO₃: 0.08 g
Actual mass naHCO₃: 0.48 g

Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.

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-==Entry title==
+==Objectives==
-* Insert content here...
+* Remake 2.5 mM luminol stock solution and 2.3 μL HRP solution to continue HRP chemiluminescence assay.
+*
+==Luminol Stock Solution in Carbonate Buffer==
+* Please refer to [[User:Melissa_Novy/Notebook/CHEM-571/2012/09/19|entry on 2012/09/19]] for luminol stock solution protocol and calculations.
+* Actual mass luminol: 0.00443 g
+* Actual concentration luminol: 2.5 mM
+* Actual mass Na<sub>2</sub>CO<sub>3</sub>: 0.08 g
+* Actual mass naHCO<sub>3</sub>: 0.48 g
+==Horseradish Peroxidase Chemiluminescence Assay==
+* Note that the HRP solution was further diluted in an attempt to slow the reaction rate such that the reaction progress could be observed via fluorescence.
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 [[category:OWWLabNotebookV1]]