User:Melissa Novy/Notebook/CHEM-571 - Revision history

Melissa Novy: /* Gold Nanoparticles */

2012-11-27T01:30:00Z

Gold Nanoparticles

←Older revision		Revision as of 01:30, 27 November 2012
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	==Gold Nanoparticles==		==Gold Nanoparticles==
	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.		* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.
-	* A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with ~~one of three~~ primers and underwent protein expression.	+	* A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with K110A forward and reverse primers and underwent protein expression using <i>E. coli</i>.
	* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis and atomic absorption spectroscopy.		* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis and atomic absorption spectroscopy.
	** AuNPs were also synthesized with ADA and horseradish peroxidase (HRP) using a similar protocol and also analyzed with UV-vis and AAS.		** AuNPs were also synthesized with ADA and horseradish peroxidase (HRP) using a similar protocol and also analyzed with UV-vis and AAS.

Melissa Novy at 01:27, 27 November 2012

2012-11-27T01:27:33Z

←Older revision		Revision as of 01:27, 27 November 2012
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	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.		* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.
	* A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with one of three primers and underwent protein expression.		* A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with one of three primers and underwent protein expression.
-	* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis spectroscopy.	+	* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis and atomic absorption spectroscopy.
		+	** AuNPs were also synthesized with ADA and horseradish peroxidase (HRP) using a similar protocol and also analyzed with UV-vis and AAS.

	==Protein Activity Assays==		==Protein Activity Assays==
-	* Two activity assays of ~~horseradish peroxidase (~~HRP) were optimized and the activity of HRP was calculated from the data. The first activity assay used aminoantipyrine (AAP) as the substrate and the reaction was monitored with UV-vis spectroscopy. The second activity assay monitored the fluorescence of luminol in the presence of HRP.	+	* Two activity assays of HRP were optimized and the activity of HRP was calculated from the data. The first activity assay used aminoantipyrine (AAP) as the substrate and the reaction was monitored with UV-vis spectroscopy. The second activity assay monitored the fluorescence of luminol in the presence of HRP.
		+	* An activity assay of ADA by measuring the absorbance of adenosine or inosine in solution is being optimized. The ultimate goal is to conduct the assay on ADA that has been conjugated to AuNPs to determine whether the activity of ADA is hindered by AuNPs.

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Melissa Novy: /* Gold Nanoparticles */

2012-11-12T20:27:45Z

Gold Nanoparticles

←Older revision		Revision as of 20:27, 12 November 2012
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	\|colspan="2"\|		\|colspan="2"\|
	==Gold Nanoparticles==		==Gold Nanoparticles==
-	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.	+	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy and atomic absorption spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase (ADA) or lysozyme rather than BSA.
-	* ~~Protein~~ expression ~~using~~ ADA ~~will be carried out~~ in the ~~near future~~.	+	* A Bradford assay was used to determine the amount of ADA obtained from protein expression. ADA was also mutated with one of three primers and underwent protein expression.
		+	* AuNPs were synthesized with lysozyme at various mole ratios of Au/lysozyme and their presence in solution was analyzed with UV-vis spectroscopy.
		+
		+	==Protein Activity Assays==
		+	* Two activity assays of horseradish peroxidase (HRP) were optimized and the activity of HRP was calculated from the data. The first activity assay used aminoantipyrine (AAP) as the substrate and the reaction was monitored with UV-vis spectroscopy. The second activity assay monitored the fluorescence of luminol in the presence of HRP.

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Melissa Novy: /* Notes */

2012-09-11T18:17:03Z

Notes

←Older revision		Revision as of 18:17, 11 September 2012
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	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.		* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.
	* Protein expression using ADA will be carried out in the near future.		* Protein expression using ADA will be carried out in the near future.
-
-	~~==Notes==~~
-	* Place some notes here for visitors
-
-	* Example: This project is currently on hold until further notice.
-

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Melissa Novy: /* Gold Nanoparticles */

2012-09-11T18:16:47Z

Gold Nanoparticles

←Older revision		Revision as of 18:16, 11 September 2012
Line 15:		Line 15:
	==Gold Nanoparticles==		==Gold Nanoparticles==
	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.		* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.
		+	* Protein expression using ADA will be carried out in the near future.

	==Notes==		==Notes==

Melissa Novy: /* Project Description/Abstract */

2012-09-05T18:28:05Z

Project Description/Abstract

←Older revision		Revision as of 18:28, 5 September 2012
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-	==~~Project Description/Abstract~~==	+	==Gold Nanoparticles==
-	* ~~Place short description of project or notes regarding this project~~	+	* Research on gold nanoparticles (AuNPs) is being continued from the previous year. AuNPs were synthesized with bovine serum albumin (BSA) in H<sub>2</sub>O at various mole ratios of Au/BSA and their presence in solution was analyzed with UV-vis spectroscopy. Different methods of synthesizing AuNPs are being studied, including using adenosine deaminase or lysozyme rather than BSA.
-		+
-	* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. ~~Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis~~ in~~, pulvinar sed, molestie a, quam. Vestibulum~~ at ~~pede. In~~ in ~~sem eget odio eleifend placerat~~. ~~Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna~~, ~~fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci~~.	+

	==Notes==		==Notes==

Melissa Novy at 14:35, 29 August 2012

2012-08-29T14:35:07Z

←Older revision		Revision as of 14:35, 29 August 2012
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Melissa Novy: Autocreated Lab Notebook name=User:Melissa Novy/Notebook/CHEM-571, content from MediaWiki:ProjectContentDefault

2012-08-24T16:20:17Z

Autocreated Lab Notebook name=User:Melissa Novy/Notebook/CHEM-571, content from MediaWiki:ProjectContentDefault

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==Project Description/Abstract==
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