User:Megan L. Channell/Notebook/Horseradish/2013/10/23: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
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== | |||
* | |||
==Objective== | |||
#To take the spectra of the pepsin-AuNP samples made yesterday | |||
#To make new HRP and Myoglobin samples | |||
==Description== | |||
# Protein-AuNP analysis | |||
## Take a picture and note physical description of each ratio | |||
## Place 1mL of each ratio in an 1.5mL eppendorf tube. | |||
## Centrifuge for 30 minutes (Be considerate of other uses here. Act quickly, and get as many tubes in the centrifuge as you can at once.) | |||
## Take a UV-Vis spectrum of each sample (Be considerate of other users here) | |||
## After taking the spectrum, save the sample in a fresh eppy tube. | |||
# Synthesis of HRP-AuNPs and Hemoglobin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## For Myoglobin make the following solutions to reflect an Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## For HRP make the following solutions to reflect an Au:Protein ratios | |||
### 230 | |||
### 250 | |||
### 270 | |||
### 290 | |||
### 310 | |||
### 330 | |||
### 350 | |||
### 370 | |||
### 390 | |||
### 410 | |||
## Water up to 5mL | |||
==Data== | |||
*How solutions were made: | |||
[[Image:CHEM571_10.23.13_solutionstable.png]] | |||
*HRP UV-Vis | |||
[[Image:CHEM571_10.23.13_HRPUvVIS.png]] | |||
==Notes== | |||
<u>Stock Solutions</u> | |||
# Gold | |||
## 49.3 mg HAuCl<sub>4</sub>·3H<sub>2</sub>O in 50mL water ---> 2.50mM | |||
# HRP | |||
## 6.6 mg HRP in 100 mL water ---> 1.5μM | |||
# Myoglobin | |||
## 28.7mg myoglobin in 100mL water ---> 16.9μM | |||
[[Category:CHEM571]] | |||
[[Category:Horseradish peroxidase]] | |||
[[Category:pepsin]] | |||
Revision as of 18:23, 27 October 2013
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Objective
Description
Data
NotesStock Solutions
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