User:Megan L. Channell/Notebook/Horseradish/2013/10/22: Difference between revisions
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|style="background-color: #EEE"|[[Image: | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | |||
* | |||
==Objective== | |||
# Synthesize Pepsin-AuNPs for reactivity tests tomorrow | |||
# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP | |||
==Procedure== | |||
# Synthesis of HRP-AuNPs and Pepsin-AuNPs | |||
## Into test tube that will contain a total of 5mL add | |||
## Au solution such that the final concentration is 0.25mM | |||
## Protein solution such that the final concentration reflects the following Au:Protein ratios | |||
### 30 | |||
### 50 | |||
### 70 | |||
### 90 | |||
### 110 | |||
### 130 | |||
### 150 | |||
### 170 | |||
### 190 | |||
### 210 | |||
## Water up to 5mL | |||
Source: [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/22 | Dr. Hartings]] | |||
==Data== | |||
* Solutions Made: | |||
[[Image:Solutions_10.22.2013.png]] | |||
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant | |||
[[Image:CMJ_UV-VIS_solutions_10222013.png]] | |||
*Pepsin UV-Vis | |||
[[Image:11.1.2013_cmj_UVVis_Pepsin.png]] | |||
*HRP UV-Vis | |||
[[Image:CHEM571_10.23.13_HRPUvVIS.png]] | |||
==Notes== | |||
This area is for any observations or conclusions that you would like to note. | |||
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. | |||
[[Category:Course]] | |||
[[Category:Miscellaneous]] | |||
[[Category:Course]] | |||
[[Category:Miscellaneous]] | |||
Latest revision as of 23:30, 26 September 2017
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Objective
Procedure
Source: Dr. Hartings Data
NotesThis area is for any observations or conclusions that you would like to note.
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