User:Megan L. Channell/Notebook/Horseradish/2013/10/22

From OpenWetWare

< User:Megan L. Channell | Notebook | Horseradish | 2013 | 10(Difference between revisions)
Jump to: navigation, search
Current revision (15:51, 6 November 2013) (view source)
 
Line 38: Line 38:
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
[[Image:CMJ_UV-VIS_solutions_10222013.png]]
[[Image:CMJ_UV-VIS_solutions_10222013.png]]
 +
*Pepsin UV-Vis
 +
[[Image:11.1.2013_cmj_UVVis_Pepsin.png]]
 +
*HRP UV-Vis
 +
[[Image:CHEM571_10.23.13_HRPUvVIS.png]]
==Notes==
==Notes==

Current revision

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Procedure

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Source: Dr. Hartings

Data

  • Solutions Made:

Image:Solutions_10.22.2013.png

  • UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant

Image:CMJ_UV-VIS_solutions_10222013.png

  • Pepsin UV-Vis

Image:11.1.2013_cmj_UVVis_Pepsin.png

  • HRP UV-Vis

Image:CHEM571_10.23.13_HRPUvVIS.png

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




Personal tools