User:Megan L. Channell/Notebook/Horseradish/2013/10/22

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(Autocreate 2013/10/22 Entry for User:Megan_L._Channell/Notebook/Horseradish)
Current revision (15:51, 6 November 2013) (view source)
 
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
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* Insert content here...
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==Objective==
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# Synthesize Pepsin-AuNPs for reactivity tests tomorrow
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# Determine the Au:Protein ratio required for fiber formation for pepsin and HRP
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==Procedure==
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# Synthesis of HRP-AuNPs and Pepsin-AuNPs
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## Into test tube that will contain a total of 5mL add
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## Au solution such that the final concentration is 0.25mM
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## Protein solution such that the final concentration reflects the following Au:Protein ratios
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### 30
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### 50
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### 70
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### 90
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### 110
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### 130
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### 150
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### 170
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### 190
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### 210
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## Water up to 5mL
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Source: [[User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/10/22 | Dr. Hartings]]
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==Data==
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* Solutions Made:
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[[Image:Solutions_10.22.2013.png]]
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*UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant
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[[Image:CMJ_UV-VIS_solutions_10222013.png]]
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*Pepsin UV-Vis
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[[Image:11.1.2013_cmj_UVVis_Pepsin.png]]
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*HRP UV-Vis
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[[Image:CHEM571_10.23.13_HRPUvVIS.png]]
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==Notes==
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This area is for any observations or conclusions that you would like to note.
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Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
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[[Category:Course]]
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[[Category:Miscellaneous]]
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[[Category:Course]]
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[[Category:Miscellaneous]]
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Current revision

Biomaterials Design Lab Main project page
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Objective

  1. Synthesize Pepsin-AuNPs for reactivity tests tomorrow
  2. Determine the Au:Protein ratio required for fiber formation for pepsin and HRP

Procedure

  1. Synthesis of HRP-AuNPs and Pepsin-AuNPs
    1. Into test tube that will contain a total of 5mL add
    2. Au solution such that the final concentration is 0.25mM
    3. Protein solution such that the final concentration reflects the following Au:Protein ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
    4. Water up to 5mL

Source: Dr. Hartings

Data

  • Solutions Made:

Image:Solutions_10.22.2013.png

  • UV-Vis taken: gold 1:10 dilution, HRP 1:2 dilution, Pepsin Stock, BSA-HRP 1:60 supernatant

Image:CMJ_UV-VIS_solutions_10222013.png

  • Pepsin UV-Vis

Image:11.1.2013_cmj_UVVis_Pepsin.png

  • HRP UV-Vis

Image:CHEM571_10.23.13_HRPUvVIS.png

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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