User:Megan L. Channell/Notebook/Horseradish/2013/09/04

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Adenosine and Inosine UV-Vis and Analysis

Objective

  • Collect the data on the UV-Vis from the ionisine dilutions

The inosine samples came from yesterday's protocol

  • Calculate calibration curves for Adenosine and Inosine
  • Run a data regressions on the class' data

Protocol

Yesterday, our adenosine dilutions were much lower than the class' average, so we redid the adenosine samples. A new stock solutions was made using yesterday's protocol and the stock solutions was made from .0809g of adenosine.

Data

A UV-Vis spectra of the new adenosine samples and the inosine samples were collected. The parameters for the UV-Vis were measured between 450 and 200nm. The peak for the insonie spectra was at 249nm while the adenosine was 259nm. This wavelength was used when calculating the calibration curve.

File:Image:Adenonsinefixedtrial2 9 4 2013 cmj.png

Matt Hartings In your Adenosine trial 2 data, all of your spectra have A values less than 0 (baseline) toward the end of the spectrum. These should be zero. You should correct this data for that (add the value that it is below to all of your A values), and make a new calibration curve. In fact, your re-corrected data might change the class's calibration curve a bit. You guys should check this.

Data

  • The Grubbs test was calculated using the formula

G=|mean-x|÷std. dev.

  • The data that our group collected from 9/3/2013 was lower. We remade Adenosine concentrations from a new stock solution, but did not do that for inosine, thus our group (5) data is not recorded.


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