User:Megan L. Channell/Notebook/Horseradish/2013/09/04: Difference between revisions
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==Protocol== | ==Protocol== | ||
Yesterday, our adenosine dilutions were much lower than the class' average, so we redid the adenosine samples. A new stock solutions was made using [[User:Megan_L._Channell/Notebook/Horseradish/2013/09/03 | yesterday's]] protocol and the stock solutions was made from .0809g of adenosine. | Yesterday, our adenosine dilutions were much lower than the class' average, so we redid the adenosine samples. A new stock solutions was made using [[User:Megan_L._Channell/Notebook/Horseradish/2013/09/03 | yesterday's]] protocol and the stock solutions was made from .0809g of adenosine. | ||
[[Image:Adenosine2 9 4 2013 cmj.jpg]] | [[Image:Adenosine2 9 4 2013 cmj.jpg]] | ||
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[[Image:Inosine 9 4 2013 cmj UVVIS.jpg]] | [[Image:Inosine 9 4 2013 cmj UVVIS.jpg]] | ||
[[Image:Inosine 9 4 2013 cmj cali.jpg]] | [[Image:Inosine 9 4 2013 cmj cali.jpg]] | ||
[[Image:Adenonsinefixedtrial2 9 4 2013 cmj.png]] | |||
[[Image:Adenosinetrial2 9 4 2013 conc.jpg]] | |||
==Data== | |||
[[Image:Adenosine 9 4 2013 cmj trial2 grubbs.png]] | |||
[[Image:Inosine 9 4 2013 cmj grubbs.png|1050px]] | |||
*The Grubbs test was calculated using the formula | |||
G=|mean-x|÷std. dev. | |||
*The data that our group collected from 9/3/2013 was lower. We remade Adenosine concentrations from a new stock solution, but did not do that for inosine, thus our group (5) data is not recorded. | |||
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Revision as of 10:01, 17 September 2013
 Biomaterials Design Lab
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Adenosine and Inosine UV-Vis and AnalysisObjective
The inosine samples came from yesterday's protocol
ProtocolYesterday, our adenosine dilutions were much lower than the class' average, so we redid the adenosine samples. A new stock solutions was made using yesterday's protocol and the stock solutions was made from .0809g of adenosine.
DataA UV-Vis spectra of the new adenosine samples and the inosine samples were collected. The parameters for the UV-Vis were measured between 450 and 200nm. The peak for the insonie spectra was at 249nm while the adenosine was 259nm. This wavelength was used when calculating the calibration curve.
Data
G=|mean-x|÷std. dev.
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