User:Megan L. Channell/Notebook/Horseradish/2013/09/03: Difference between revisions
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A total of 14 dilutions were made | A total of 14 dilutions were made | ||
# Using the formula: M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub>, the amount of stock solution needed was found. | # Using the formula: M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub>, the amount of stock solution needed was found. | ||
# The sample of stock solution needed was pipetted into a 10mL volumetric flask and diluted with DI water | # The sample of stock solution needed was pipetted into a 10mL volumetric flask and diluted with DI water | ||
# This process was repeated 14 times | # This process was repeated 14 times | ||
A sample calculation: Amount of Stock Solution.= [10 mL(dilution concentration)/(stock solution concentration)] | |||
*[[Image:Cmj conc 9 3.jpg]] | *[[Image:Cmj conc 9 3.jpg]] | ||
Revision as of 14:17, 4 September 2013
 Biomaterials Design Lab
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Adenosine and Inosine UV-VisObjectiveFinding the molar absorptivities of adenosine and inosine. This will help later on in studying adenosine deaminase (ADA) which converts adenosine to inosine.. The structural differences between the two molecules is that adenosine has a primary amine while inosine has a carboxy group. ProtocolStock Solutions Two different stocks solutions were made, one adenosine and one inosine
Dilutions A total of 14 dilutions were made
A sample calculation: Amount of Stock Solution.= [10 mL(dilution concentration)/(stock solution concentration)]
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