User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/04/08: Difference between revisions

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==Entry title==
==April 8, 2014==
* Insert content here...
===Objectives===
*Centrifuge 2nd procedure Ag:BSA NPs
*UVvis of Ag:BSA NPs from last time
*Put dry-autoclaved scaffolds into bacteria
*Make Au nanofibers and print scaffolds (12) for brute force
 
===Sample Prep: Nanofibers for Brute Force===
[[Image:2014_0211_myo_bsa_NPs.PNG]]
*Solutions:
**Au: 0.011g in 10mL to make 2.793mM solution
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution
====Brute Force Nanofiber-coated Scaffolds====
*4 PLA scaffolds printed for each test tube
*Gold:Myoglobin (100:1) nanofibers synthesized by above method
*Nanofibers dried onto PLA scaffolds
*Scaffolds submerged in water
*Autoclaved
 
===Results: UV-vis===
[[Image:2014 0408 abs AgBSA NPs.PNG]]
*Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm
 
===Sample Prep: Scaffolds in Bacteria===




Latest revision as of 23:54, 26 September 2017

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April 8, 2014

Objectives

  • Centrifuge 2nd procedure Ag:BSA NPs
  • UVvis of Ag:BSA NPs from last time
  • Put dry-autoclaved scaffolds into bacteria
  • Make Au nanofibers and print scaffolds (12) for brute force

Sample Prep: Nanofibers for Brute Force

  • Solutions:
    • Au: 0.011g in 10mL to make 2.793mM solution
    • Myoglobin: 0.011g in 10mL to make 0.0621mM solution

Brute Force Nanofiber-coated Scaffolds

  • 4 PLA scaffolds printed for each test tube
  • Gold:Myoglobin (100:1) nanofibers synthesized by above method
  • Nanofibers dried onto PLA scaffolds
  • Scaffolds submerged in water
  • Autoclaved

Results: UV-vis

  • Absorbance peaks were not as expected, as Ag NPs are supposed to peak around 400nm

Sample Prep: Scaffolds in Bacteria


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