User:Megan L. Channell/Notebook/3D printing scaffolds with Au nanofibers/2014/04/01: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ===Objectives=== | ||
* | *Making new scaffolds | ||
*New AgNP procedures | |||
===Au:Myoglobin Nanofibers: Sample Prep for Cell Culture=== | |||
[[Image:2014_0211_myo_bsa_NPs.PNG]] | |||
*Solutions: | |||
**TRIS dopamine: 0.0475g dopamine hydrochloride and 0.03g TRIS in 25mL to make 10mM solution at pH 8.46 | |||
**Au: 0.011g in 10mL to make 2.793mM solution | |||
**Myoglobin: 0.011g in 10mL to make 0.0621mM solution | |||
====Plain Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Three test tubes filled with scaffolds and water | |||
*Autoclaved | |||
====Brute Force Nanofiber-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above method | |||
*Nanofibers dried onto PLA scaffolds | |||
*Scaffolds submerged in water | |||
*Autoclaved | |||
====Original Method for Nanofiber-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with scaffolds in solution | |||
*Autoclaved | |||
====Polydopamine-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Scaffolds submerged in 10mM pH 8.46 TRIS dopamine hydrochloride solution for 1 hour | |||
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours | |||
*Rinse with deionized water and allow scaffold to dry overnight | |||
*Scaffolds submerged in water | |||
*Autoclaved | |||
====Polydopamine Nanofiber-coated Scaffolds==== | |||
*4 PLA scaffolds printed for each test tube | |||
*Scaffolds submerged in 10mM pH 8.5 TRIS dopamine hydrochloride solution for 1 hour | |||
*Add 1mL of 0.0621 Myoglobin to each test tube, shake tube to combine, allow surface functionalization to occur for 5 hours | |||
*Rinse with deionized water and allow scaffold to dry overnight | |||
*Gold:Myoglobin (100:1) nanofibers synthesized by above procedure with polydopamine-coated scaffolds in solution | |||
*Autoclaved | |||
===Breakdown=== | |||
=====Today===== | |||
*Time sensitive: Make 40mL TRIS-polydopamine solution | |||
*Time sensitive: Make 6 tests tubes of TRIS-polydopamine solution, put scaffolds in all 6 test tubes at '''12 p.m.''' (3 for pD only, 3 for pD-original) | |||
*Make Au and Myoglobin solutions | |||
*Make 9 test tubes of 100:1 Au:Myo Nanofibers (3 for original, 3 for brute force fibers, 3 for pD-original tomorrow; see volumes in chart above) | |||
*Time sensitive: At '''1 p.m.''' add 1mL of 0.0621M Myoglobin to all 6 tubes (3 for pD only, 3 for pD-original) | |||
*Put scaffolds into 3 of the 9 Au:Myo test tubes when finished (3 for original) | |||
*Make 3 test tubes of water -> put scaffolds into 3 test tubes when finished | |||
*Put 9 test tubes (3 brute force, 3 original, 3 water) into oven, set for 4 hours at 80C | |||
*Time Sensitive: At '''6 p.m.''' rinse pD scaffolds that have soaked for 5 hours, submerge 6 in water overnight (if possible, submerge 3 of 6 tubes in Au:Myo solution and put all 6 in oven for 4 hours), dump Au:Myo fibers onto scaffolds for brute force | |||
=====Tomorrow===== | |||
*If oven was not finished yesterday: submerge 3 pD scaffolds into original solution, leave other 3 in water and put in oven for 4 hours at 80C | |||
*Submerged dried brute force scaffolds in water | |||
*Autoclave all: 3 plain, 3 brute force, 3 original, 3 pD-Myo, 3 pD-Myo-Au | |||
===Sample Preparation: AgNPs=== | |||
=====Without BSA===== | |||
*From [http://pubs.acs.org/doi/pdf/10.1021/ed084p322 Synthesis and Study of Silver Nanoparticles] | |||
*10 ml of 1 mM AgNO3 was added dropwise (about 1 drop/sec) to 30 ml of 2 mM NaBH4 (cooled in ice bath), while the reaction mixture was vigorously stirred using a magnetic stir plate. Entire procedure takes about 3 minutes. | |||
===Sample Preparation: Bacteria Culture=== | |||
*Make solution | |||
*Autoclave | |||
Latest revision as of 23:54, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
Au:Myoglobin Nanofibers: Sample Prep for Cell Culture
Plain Scaffolds
Brute Force Nanofiber-coated Scaffolds
Original Method for Nanofiber-coated Scaffolds
Polydopamine-coated Scaffolds
Polydopamine Nanofiber-coated Scaffolds
BreakdownToday
Tomorrow
Sample Preparation: AgNPsWithout BSA
Sample Preparation: Bacteria Culture
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