User:Mauricio Bedoya/Notebook/Project 1/2008/05/03: Difference between revisions

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* expD
* expD
** 2000fps
** 2000fps
** Half concentration HA
* expE
** 200fps
** Half concentration HA
** Half concentration HA


Revision as of 20:02, 3 May 2008

Microrheology properties of Hyaluronan <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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First HA experiments

05/02

  • Added 200ul of buffer to 1mg of HA (5mg/mL)
  • Rehydrated 500nm fluorescent red particles on buffer
  • used 100÷1000ul pippetor

05/03

  • prepared a sample solution of HA (.5mg/mL)
    • 5ul of HA sample
    • 1ul of beads
    • 94ul of buffer
    • number of particles seems ok in the movie (not too cluttered, but enough)
  • prepared a half concentration solution
    • 50ul of previous soln
    • 50ul of buffer
  • expA
    • 2000fps
    • 497us of expt
    • 2100 graylevels
    • 90x magnification
    • some tracers stuck to the base of glass
  • expB
    • similar conditions to expA
    • slightly different illumination
    • some aggregates are observed and some dust
  • expC
    • 200fps
  • expD
    • 2000fps
    • Half concentration HA
  • expE
    • 200fps
    • Half concentration HA

First HA experiments: particle analysis

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