User:Maureen McKeague: Difference between revisions

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'''Maureen McKeague'''
'''Maureen McKeague'''
<br>[http://www.stanford.edu/ Stanford University]
<br>[http://www.stanford.edu/ Stanford University]
<br>Shriram Center, Room 244
<br>Shriram Center, Room 256
<br> Mail Code 4245
<br> Mail Code 4245
<br>443 Via Ortega
<br>443 Via Ortega
Line 17: Line 17:


==Research interests==
==Research interests==
My past, current, and future research aims to improve the workflow of the selection and characterization of small molecule-binding aptamers towards the ultimate goal of developing both in vitro and in vivo biosensors that link important metabolite concentrations to readily detectable signals for high throughput screening and diagnostics.
I am passionate about improving human health and quality of life using functional nucleic acids. Specifically, my past, current & future research aims to improve the selection and characterization of small molecule-binding aptamers and ribozymes towards the ultimate goal of developing both in vitro and in vivo biosensors for high-throughput drug screening and disease diagnosis.


==Publications==
==Publications==
<!-- Replace the PubMed ID's ("pmid=#######") below with the PubMed ID's for your publications.  You can add or remove lines as needed -->
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#McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology, Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16. <i> in press </i>
#Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. [http://pubs.acs.org/doi/pdf/10.1021/acscombsci.5b00163 Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing]. ACS Comb Sci. 2016.
#McKeague, M.; Wong, R.S.; Smolke, C.D. [http://nar.oxfordjournals.org/content/early/2016/03/10/nar.gkw151.abstract?keytype=ref&ijkey=P6DDJUKcmpn8XIM Opportunities in the design and application of RNA for gene expression control] NAR, 2016.
#McKeague, M.; Wang, Y-H.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/acschembio.5b00518 In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control] ACS Chem. Biol, 2015, 10 (11), 2463–2467.
#McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. [http://link.springer.com/article/10.1007%2Fs00239-015-9708-6 Analysis of In Vitro Aptamer Selection Parameters]. J Mol Evol. 2015,  81(5), 150-161.
#Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. [http://pubs.rsc.org/en/content/articlelanding/2015/an/c5an01075f#!divAbstract An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers], Analyst 2015, 140 (8) 6643–6651.
#McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.[http://pubs.acs.org/doi/abs/10.1021/acs.analchem.5b02102 Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation], Analytical Chemistry, 2015, 87 (17), 8608–8612.
#Chang, A.L.; McKeague, M.; Smolke, C.D. [http://www.sciencedirect.com/science/article/pii/B9780128011225000192 Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance] In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
#McKeague, M.; DeRosa, M.C. [http://smithandfranklin.com/current-issues/Aptamers-and-SELEX-Tools-for-the-Development-of-Transformative-Molecular-Recognition-Technology%20/12/6/38/html Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology], Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.  
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
#McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. [http://www.mdpi.com/2072-6651/6/8/2435 Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A], Toxins, 2014, 6 (8), 2435-2452.
#Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/ac5001527 Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform], Analytical Chemistry, 2014, 86 (7), 3273–3278.
#Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. [http://pubs.acs.org/doi/abs/10.1021/ac5001527 Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform], Analytical Chemistry, 2014, 86 (7), 3273–3278.
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#McKeague, M.; DeRosa, M.C. [http://www.hindawi.com/journals/jna/2012/748913/cta/ Challenges and Opportunities for Small Molecule Aptamer Development], Journal of Nucleic Acids, 2012.  
#McKeague, M.; DeRosa, M.C. [http://www.hindawi.com/journals/jna/2012/748913/cta/ Challenges and Opportunities for Small Molecule Aptamer Development], Journal of Nucleic Acids, 2012.  
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. Database: Journal of Biological Databases and Curation. 2012.   
#Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. [http://database.oxfordjournals.org/content/2012/bas006.full Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments]. Database: Journal of Biological Databases and Curation. 2012.   
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in Aptamer-Based Biosensors for Food Safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech.
#McKeague, M.; Giamberardino, A.; DeRosa, M.C. [http://www.intechopen.com/articles/show/title/advances-in-aptamer-based-biosensors-for-food-safety Advances in Aptamer-Based Biosensors for Food Safety], Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column]. Food Chem. 2011, 127 (3), 1378-1384.
#De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. [http://www.sciencedirect.com/science/article/pii/S0308814611002147 Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column]. Food Chem. 2011, 127 (3), 1378-1384.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers]. Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
#McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. [http://www.mdpi.com/1422-0067/11/12/4864/ Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers], Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers]. RNA. 2010, 16 (11), 2252-2262.
#Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. [http://rnajournal.cshlp.org/content/16/11/2252.long Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers], RNA. 2010, 16 (11), 2252-2262.


==Useful links==
==Useful links==
*New search engine for aptamers! [http://aptamerbase.semanticscience.org/ Aptamer Base]
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance]
*Dance Your PhD 2010 [https://vimeo.com/14528924/ SELEX Dance]
*My [http://www.linkedin.com/pub/maureen-mckeague/26/b59/645/ LinkedIn] profile
*My [http://www.linkedin.com/in/maureenmckeague/ LinkedIn] profile
*[[Media:CVMCKEAGUE.pdf|Full curriculum vitae]]
*[http://www.oligotherapeutics.org/interview-with-maureen-mckeague-phd/ Interview with the Oligonucleotide Therapeutics Society]
*[http://aptamersociety.org/about-insoap/ International Society on Aptamers]
*Building a search engine for aptamers! [http://www.freebase.com/base/aptamer Aptamer Base]
*2015 Analytical Chemistry [http://pubs.acs.org/iapps/liveslides/pages/index.htm?mscNo=acs.analchem.5b02102 LiveSlides ]

Revision as of 15:25, 26 April 2016

Contact Info

Maureen McKeague

Maureen McKeague
Stanford University
Shriram Center, Room 256
Mail Code 4245
443 Via Ortega
Stanford, CA 94305
Email me through OpenWetWare

I work in the Smolke lab

Education

Research interests

I am passionate about improving human health and quality of life using functional nucleic acids. Specifically, my past, current & future research aims to improve the selection and characterization of small molecule-binding aptamers and ribozymes towards the ultimate goal of developing both in vitro and in vivo biosensors for high-throughput drug screening and disease diagnosis.

Publications

  1. Valenzano, S.; De Girolamo, A.; DeRosa, M.C.; McKeague, M.; Schena, R.; Catucci, L.; Pascale, M. Screening and Identification of DNA Aptamers to Tyramine Using in Vitro Selection and High-Throughput Sequencing. ACS Comb Sci. 2016.
  2. McKeague, M.; Wong, R.S.; Smolke, C.D. Opportunities in the design and application of RNA for gene expression control NAR, 2016.
  3. McKeague, M.; Wang, Y-H.; Smolke, C.D. In Vitro Screening and in Silico Modeling of RNA-Based Gene Expression Control ACS Chem. Biol, 2015, 10 (11), 2463–2467.
  4. McKeague, M.; McConnell, E.M.; Cruz-Toledo, J.; Bernard, E.D.; Pach, A.; Zhang, X.; Beking, M.; Francis, T.; Giamberardino, A.; Cabecinha, A.; Ruscito, A.; Aranda-Rodriguez, R.; Dumontier, M.; DeRosa, M.C. Analysis of In Vitro Aptamer Selection Parameters. J Mol Evol. 2015, 81(5), 150-161.
  5. Frost, N.R.; McKeague, M.; Falcioni, D.; DeRosa, M.C. An in Solution Assay For Parallel Interrogation of Structure and Affinity of Small Molecule-Binding Aptamers, Analyst 2015, 140 (8) 6643–6651.
  6. McKeague, M.; De Girolamo, A.; Valenzano, S.; Pascale, M.; Ruscito, A.; Ranganathan, V.; Frost, N.; Hill, K.; Smith, M.; McConnell, E.; DeRosa, M.C.Comprehensive Analytical Comparison of Strategies Used for Small Molecule Aptamer Evaluation, Analytical Chemistry, 2015, 87 (17), 8608–8612.
  7. Chang, A.L.; McKeague, M.; Smolke, C.D. Facile characterization of aptamer kinetic and equilibrium binding properties using surface plasmon resonance In: Burke-Aguero, D., editor. Methods in Enzymology. 2014, 549, 451-466.
  8. McKeague, M.; DeRosa, M.C. Aptamers and SELEX: Tools for the Development of Transformative Molecular Recognition Technology, Aptamers and Synthetic Antibodies, 2014, 1 (1), 12-16.
  9. McKeague, M.; Ranganathan, V.; Hill, K.; Bardozy, V.; Mezaros, T.; DeRosa, M.C. Selection and Characterization of a Novel DNA Aptamer For Label-Free Fluorescence Biosensing of Ochratoxin A, Toxins, 2014, 6 (8), 2435-2452.
  10. Chang A.L.; McKeague, M.; Liang, J.C.; Smolke, C.D. Kinetic and Equilibrium Binding Characterization of Aptamers to Small Molecules using a Label-Free, Sensitive, and Scalable Platform, Analytical Chemistry, 2014, 86 (7), 3273–3278.
  11. Mastronardi, E.; McKeague, M.; Monreal, C.; DeRosa, M.C. Development and Application of Crop Exudate Specific Aptamers, Journal of Biomolecular Structure and Dynamics, 2013, 31 (1), 89.
  12. McKeague, M.; Foster, A.; Miguel, Y.; Giamberardino, A.; Verdin, C.; Chan, J.Y.; DeRosa, M.C. Development of a DNA aptamer for direct and selective homocysteine detection in human serum, RSC Advances, 2013, 3 (46), 24415-24422.
  13. McKeague, M.; DeRosa, M.C. Challenges and Opportunities for Small Molecule Aptamer Development, Journal of Nucleic Acids, 2012.
  14. Cruz-Toledo, J.; McKeague, M.; Zhang, X.; Giamberardino, A.; McConnell, E.; Francis, T.; DeRosa, M.C.; Dumontier, M. Aptamer Base: A collaborative knowledge base to describe aptamers and SELEX experiments. Database: Journal of Biological Databases and Curation. 2012.
  15. McKeague, M.; Giamberardino, A.; DeRosa, M.C. Advances in Aptamer-Based Biosensors for Food Safety, Environmental Biosensors. 2011, Vernon Somerset (Ed.) ISBN: 9789533074863, InTech. DOI:10.5772/22350
  16. De Girolamo, A.; McKeague, M.; Miller, J.D.; DeRosa, M.C.; Visconti, A. Determination of Ochratoxin A in Wheat After Clean-Up through a DNA Aptamer-Based Solid Phase Extraction Column. Food Chem. 2011, 127 (3), 1378-1384.
  17. McKeague, M.; Bradley, C.R.; De Girolamo, A.; Visconti, A.; Miller, J.D.; Derosa, M.C. Screening and Initial Binding Assessment of Fumonisin B(1) Aptamers, Int. J. Mol. Sci. 2010, 11 (12), 4864-4881.
  18. Luo, X.; McKeague, M.; Pitre, S.; Dumontier, M.; Green, J.; Golshani, A.; Derosa, M.C.; Dehne, F. Computational Approaches Toward the Design of Pools for the in Vitro Selection of Complex Aptamers, RNA. 2010, 16 (11), 2252-2262.

Useful links

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