Objective
To perform Ocean Optics on Proteinase K at 10 nM concentration,
Description
For instructions on the Ocean Optics protocol see 9/9/15.
- Preparing AuNP fiber samples
- Five 1 mL predried fiber samples were obtained
- 0.2mL of 100mM Tris and 50mM CaCl2 pH 8 buffer was added into the first eppendorf tube of fiber samples
- This was continued for all five samples
- All five samples were combined into the cuvette to run with the ocean optics
- Preparing Protinase K solution
- Proteinase K tube 1G was mixed with 1 mL of 100mM Tris and 50mM CaCl2 pH 8 buffer
- Proteinase K conentration: (0.00154g)*(1mol/28,900g)*(1/0.001L)= 0.0000532 M Protinase K
- Proteinase K dilution: (53278 nM)*(0.1 mL Proteinase K) = (x nM)*(10 mL total dilution) => x = 532 nM
- Proteinase K dilution: 0.1 mL of proteinase k solution was mixed with 9.9 mL of buffer
- Amount of Proteinase K solution needed for 3mL with 1μM concentration: M1*V1 = M2*V2 => (532M)*(V1) = (10 nM)*(3 mL) => V1 = 56.3 μL Protinase K
- Amount of Buffer solution need to get to 3mL: (3mL total)-(0.056 mL Protinase K solution) - 1mL fibers = 1.940 mL buffer
- The Protinase K solution, AuNP fiber samples, and buffer were mixed in a cuvette and run through the Ocean Optics
Results
NEEDS WORKING UP
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