User:Matthew R Skorski/Notebook/471 - Exp BioChem/2015/09/08: Difference between revisions

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[[Image:UVVis 08 CHEM671 Dilution Practice.png|600px]]
[[Image:UVVis 08 CHEM671 Dilution Practice.png|600px]]


[[User:Matt Hartings|Matt Hartings]] What can you say about this graph? What is your molar absorptivity in terms of M<sup>-1</sup> cm<sup>-1</sup>? What can you say about that errant point? Can you use any quantitative analysis jiujitsu to get rid of that point? Or does it have to stay?


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Revision as of 19:02, 22 September 2015

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=Objective

Learn how to use the UV-VIS and Fluorescence machines.

Description

The overall procedure can be found at Dr. Harting's notebook. The major goal was to make dilutions from a stock solution of lysozyme with each group being assigned a different dilution. The dilutions were used to make a calibration curve for UV-VIS.


  1. Dilute 21.4μM lysozyme to a 1/10 dilution
    1. Wanted 3mL total volume
      1. lysozyme calculations: 3mL*(1/10) = 0.3mL lysozyme
      2. water calculations: 3*(9/10) = 2.7mL water

Matt Hartings How did you make all of the dilutions that you used?

Calibration curve



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