User:Matt Hartings/Notebook/Photosynthesis/2015/11/10: Difference between revisions
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==Objective== | ==Objective== | ||
Learn how to maintain an OpenWetWare Notebook. | Learn how to maintain an OpenWetWare Notebook. | ||
<u>Sample 1</u> | |||
Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans | |||
# Stock solutions: | |||
## KCl | |||
### 15mg KCl lyophilized from Tris | |||
## OPD | |||
### 3.4mg OPD in 3.4mL 1-pentanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL 1-pentanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 200 scan average | |||
### Perform a scan every 10 seconds | |||
### Stop after 10minutes | |||
## Files saved as: Mb_OPD_H2O2_1pentanol_SCANNUMBER.txt | |||
## 0.225mL KCl suspension added after 3 scans | |||
## Should see this in the spectra | |||
<u>Sample 2</u> | |||
Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans | |||
# Stock solutions: | |||
## Myoglobin | |||
### 15mg lyophilized Tris KCl | |||
## OPD | |||
### 3.7mg OPD in 3.7mL 1-pentanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL 1-pentanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 200 scan average | |||
### Perform a scan every 10 seconds | |||
### Stop after 10minutes | |||
## Files saved as: Mb_OPD_H2O2_1pentanol_SCANNUMBER.txt | |||
## 0.225mL KCl solution added after 5 scans | |||
## Should see this in the spectra | |||
Revision as of 08:44, 10 November 2015
Protein Re-engineering | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> |
ObjectiveLearn how to maintain an OpenWetWare Notebook. Sample 1 Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
Sample 2 Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
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