User:Matt Hartings/Notebook/Photosynthesis/2015/01/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
|style="background-color: #EEE"|[[Image:Hartings_AU_Photosynthesis_Lab_Header.png|128px]]<span style="font-size:22px;"> Protein Re-engineering</span>
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==Objective==
==Objective==
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Making a new batch of myoglobin lyophilized from KCl and phosphate
==Procedure==
# Measured out 4.8mg of myoglobin and placed in a 50mL falcon tube
# Measured out 0.498g of KCl and placed in the 50mL falcon tube
# Added 20mL of 10mM phosphate buffer (pH 7) to the falcon tube
# Freezing sample in dry ice (we're out of liquid nitrogen at the moment)
# Add to lyophilizer


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Latest revision as of 00:40, 27 September 2017

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Objective

Making a new batch of myoglobin lyophilized from KCl and phosphate

Procedure

  1. Measured out 4.8mg of myoglobin and placed in a 50mL falcon tube
  2. Measured out 0.498g of KCl and placed in the 50mL falcon tube
  3. Added 20mL of 10mM phosphate buffer (pH 7) to the falcon tube
  4. Freezing sample in dry ice (we're out of liquid nitrogen at the moment)
  5. Add to lyophilizer


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