User:Matt Hartings/Notebook/Photosynthesis/2014/12/09: Difference between revisions
From OpenWetWare
No edit summary |
|||
Line 25: | Line 25: | ||
### 2.6mg OPD in 2.6mL water | ### 2.6mg OPD in 2.6mL water | ||
## H<sub>2</sub>O<sub>2</sub> | ## H<sub>2</sub>O<sub>2</sub> | ||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL | ### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water | ||
#Experimental Run | #Experimental Run | ||
## Sample chamber set to 25C and to stir | ## Sample chamber set to 25C and to stir | ||
Line 50: | Line 50: | ||
### 2.5mg OPD in 2.5mL water | ### 2.5mg OPD in 2.5mL water | ||
## H<sub>2</sub>O<sub>2</sub> | ## H<sub>2</sub>O<sub>2</sub> | ||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL | ### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water | ||
#Experimental Run | #Experimental Run | ||
## Sample chamber set to 25C and to stir | ## Sample chamber set to 25C and to stir | ||
Line 63: | Line 63: | ||
## Files saved as: KCl_OPD_H2O2_tris_water_25_SCANNUMBER.txt | ## Files saved as: KCl_OPD_H2O2_tris_water_25_SCANNUMBER.txt | ||
## 0.225mL KCl solution added after 3 scans | ## 0.225mL KCl solution added after 3 scans | ||
## Should see this in the spectra | |||
<u>Sample 3</u> | |||
Mb lyophilized from Tris and KCl in methanol. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans | |||
# Stock solutions: | |||
## Myoglobin | |||
### 15mg myoglobin from Tris KCl in 1mL methanol | |||
## OPD | |||
### 2.4mg OPD in 2.4mL methanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 200 scan average | |||
### Perform a scan every 10 seconds | |||
### Stop after 10minutes | |||
## Files saved as: Mb_OPD_H2O2_tris_meoh_25_SCANNUMBER.txt | |||
## 0.225mL Mb solution added after 3 scans | |||
## Should see this in the spectra | ## Should see this in the spectra | ||
Revision as of 08:19, 9 December 2014
Protein Re-engineering | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectiveLearn how to maintain an OpenWetWare Notebook. SamplesSample 1 Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
Sample 2 lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
Sample 3 Mb lyophilized from Tris and KCl in methanol. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
|