User:Matt Hartings/Notebook/Photosynthesis/2014/12/09: Difference between revisions

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### 2.6mg OPD in 2.6mL water
### 2.6mg OPD in 2.6mL water
## H<sub>2</sub>O<sub>2</sub>
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
#Experimental Run
#Experimental Run
## Sample chamber set to 25C and to stir
## Sample chamber set to 25C and to stir
Line 50: Line 50:
### 2.5mg OPD in 2.5mL water
### 2.5mg OPD in 2.5mL water
## H<sub>2</sub>O<sub>2</sub>
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
#Experimental Run
#Experimental Run
## Sample chamber set to 25C and to stir
## Sample chamber set to 25C and to stir
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## Files saved as: KCl_OPD_H2O2_tris_water_25_SCANNUMBER.txt
## Files saved as: KCl_OPD_H2O2_tris_water_25_SCANNUMBER.txt
## 0.225mL KCl solution added after 3 scans  
## 0.225mL KCl solution added after 3 scans  
## Should see this in the spectra
<u>Sample 3</u>
Mb lyophilized from Tris and KCl in methanol. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans
# Stock solutions:
## Myoglobin
### 15mg myoglobin from Tris KCl in 1mL methanol
## OPD
### 2.4mg OPD in 2.4mL methanol
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol
#Experimental Run
## Sample chamber set to 25C and to stir
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
## 2.025mL OPD stock solution
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
### Start Data Collection
### 1ms Integration
### 200 scan average
### Perform a scan every 10 seconds
### Stop after 10minutes
## Files saved as: Mb_OPD_H2O2_tris_meoh_25_SCANNUMBER.txt
## 0.225mL Mb solution added after 3 scans
## Should see this in the spectra
## Should see this in the spectra


Revision as of 08:19, 9 December 2014

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Objective

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Samples

Sample 1

Mb lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans

  1. Stock solutions:
    1. Myoglobin
      1. 15mg myoglobin from Tris KCl
    2. OPD
      1. 2.6mg OPD in 2.6mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 200 scan average
      4. Perform a scan every 10 seconds
      5. Stop after 10minutes
    5. Files saved as: Mb_OPD_H2O2_tris_water_25_SCANNUMBER.txt
    6. 0.225mL Mb solution added after 3 scans
    7. Should see this in the spectra

Sample 2

lyophilized from Tris and KCl in water. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans

  1. Stock solutions:
    1. Myoglobin - no myoglobin this run
      1. 15mg lyophilized Tris KCl
    2. OPD
      1. 2.5mg OPD in 2.5mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 200 scan average
      4. Perform a scan every 10 seconds
      5. Stop after 10minutes
    5. Files saved as: KCl_OPD_H2O2_tris_water_25_SCANNUMBER.txt
    6. 0.225mL KCl solution added after 3 scans
    7. Should see this in the spectra

Sample 3

Mb lyophilized from Tris and KCl in methanol. scans every 10 seconds for 10 minutes. 1 scan = 1ms Integration, averaging over 200 scans

  1. Stock solutions:
    1. Myoglobin
      1. 15mg myoglobin from Tris KCl in 1mL methanol
    2. OPD
      1. 2.4mg OPD in 2.4mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 200 scan average
      4. Perform a scan every 10 seconds
      5. Stop after 10minutes
    5. Files saved as: Mb_OPD_H2O2_tris_meoh_25_SCANNUMBER.txt
    6. 0.225mL Mb solution added after 3 scans
    7. Should see this in the spectra


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