User:Matt Hartings/Notebook/Photosynthesis/2014/10/14: Difference between revisions
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== | ==Samples== | ||
<u>sample 1</u> | |||
# Stock solutions: | |||
## Myoglobin | |||
### 15.7mg Mb lyophilized from KCl/phosphate in 1mL ethanol | |||
## OPD | |||
### 2.6mg OPD in 2.6mL ethanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL ethanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: Mb_OPD_H2O2_etoh_phosphate_25_SCANNUMBER.txt | |||
## 0.225mL Mb/ethanol mix | |||
## This is denoted in the data file for the third scan | |||
<u>sample 2</u> | |||
# Stock solutions: | |||
## KCl Phosphate | |||
### 15.2mg lyophilized KCl/phosphate in 1mL methanol | |||
## OPD | |||
### 2.8mg OPD in 2.8mL methanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol | |||
#Experimental Run | |||
## Sample chamber set to 25C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt | |||
## 0.225mL KCl/phosphate in methanol | |||
## This is denoted in the data file for the third scan | |||
Revision as of 09:22, 14 October 2014
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Samplessample 1
sample 2
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