User:Matt Hartings/Notebook/Photosynthesis/2013/10/22

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/10/22 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
 
==Objective==
==Objective==
-
Learn how to maintain an OpenWetWare Notebook.
+
Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (5, 15, and 25C).
 +
 
 +
==Procedure and Data==
 +
 
 +
<u>sample 1</u>
 +
# Stock solutions:
 +
## Myoglobin
 +
### 1.6mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
 +
## OPD
 +
### 2.5mg OPD in 2.5mL water
 +
## H<sub>2</sub>O<sub>2</sub>
 +
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
 +
#Experimental Run
 +
## Sample chamber set to 5C and to stir
 +
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
 +
## 2.025mL OPD stock solution
 +
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
 +
### Start Data Collection
 +
### 1ms Integration
 +
### 10 scan average
 +
### Wait 1 minute after each scan
 +
### Stop after 2 hours
 +
## Files saved as: Mb_OPD_H2O2_phosphate_water_5_SCANNUMBER.txt
 +
## 0.225mL Mb stock solution added just before the third scan
 +
## This is denoted in the data file for the third scan

Revision as of 08:41, 22 October 2013

Protein Re-engineering Main project page
Previous entry      Next entry


Objective

Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (5, 15, and 25C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 1.6mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
    2. OPD
      1. 2.5mg OPD in 2.5mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 5C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_phosphate_water_5_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



Personal tools