User:Matt Hartings/Notebook/Photosynthesis/2013/10/17

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/10/17 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Line 11: Line 11:
==Objective==
==Objective==
-
Learn how to maintain an OpenWetWare Notebook.
+
Take kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (45C). Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (5C, 15C, and 25C).
 +
 
 +
==Procedure and Data==
 +
 
 +
<u>sample 1</u>
 +
# Stock solutions:
 +
## Myoglobin
 +
### 1.3mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL methanol
 +
## OPD
 +
### 3.6mg OPD in 3.6mL water
 +
## H<sub>2</sub>O<sub>2</sub>
 +
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
 +
#Experimental Run
 +
## Sample chamber set to 45C and to stir
 +
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette
 +
## 2.025mL OPD stock solution
 +
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution
 +
### Start Data Collection
 +
### 1ms Integration
 +
### 10 scan average
 +
### Wait 1 minute after each scan
 +
### Stop after 2 hours
 +
## Files saved as: Mb_OPD_H2O2_Acetate_meoh_45_SCANNUMBER.txt
 +
## 0.225mL Mb stock solution added just before the third scan
 +
## This is denoted in the data file for the third scan
 +
 

Revision as of 07:53, 17 October 2013

Protein Re-engineering Main project page
Previous entry      Next entry



Objective

Take kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (45C). Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (5C, 15C, and 25C).

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 1.3mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL methanol
    2. OPD
      1. 3.6mg OPD in 3.6mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 45C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Acetate_meoh_45_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



Personal tools