User:Matt Hartings/Notebook/Photosynthesis/2013/10/17: Difference between revisions
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==Objective== | ==Objective== | ||
Take kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (45C). Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in | Take kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (45C). Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (5C, 15C, and 25C). | ||
==Procedure and Data== | ==Procedure and Data== | ||
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### Stop after 2 hours | ### Stop after 2 hours | ||
## Files saved as: Mb_OPD_H2O2_phosphate_meoh_5_SCANNUMBER.txt | ## Files saved as: Mb_OPD_H2O2_phosphate_meoh_5_SCANNUMBER.txt | ||
## 0.225mL Mb stock solution added just before the third scan | |||
## This is denoted in the data file for the third scan | |||
<u>sample 3</u> | |||
# Stock solutions: | |||
## Myoglobin | |||
### No myoglobin was used in this sample | |||
## OPD | |||
### 3.6mg OPD in 3.6mL methanol | |||
## H<sub>2</sub>O<sub>2</sub> | |||
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL methanol | |||
#Experimental Run | |||
## Sample chamber set to 5C and to stir | |||
## N<sub>2</sub> run through the cuvette holder to reduce condensation on cuvette | |||
## 2.025mL OPD stock solution | |||
## 0.75mL H<sub>2</sub>O<sub>2</sub> stock solution | |||
### Start Data Collection | |||
### 1ms Integration | |||
### 10 scan average | |||
### Wait 1 minute after each scan | |||
### Stop after 2 hours | |||
## Files saved as: OPD_H2O2_phosphate_meoh_5_SCANNUMBER.txt | |||
## 0.225mL Mb stock solution added just before the third scan | ## 0.225mL Mb stock solution added just before the third scan | ||
## This is denoted in the data file for the third scan | ## This is denoted in the data file for the third scan |
Revision as of 11:14, 17 October 2013
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ObjectiveTake kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (45C). Take kinetic data for Mb (lyophilized from KCl/phosphate buffer) catalyzed o-phenylenediamine + H2O2 reaction in methanol (5C, 15C, and 25C). Procedure and Datasample 1
sample 2
sample 3
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