User:Matt Hartings/Notebook/Photosynthesis/2013/10/10

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(Procedure and Data)
Line 20: Line 20:
### 1.3mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
### 1.3mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
## OPD
## OPD
-
### 3.5mg OPD in 2.5mL water
+
### 3.5mg OPD in 3.5mL water
## H<sub>2</sub>O<sub>2</sub>
## H<sub>2</sub>O<sub>2</sub>
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water
### 0.114mL 30% H<sub>2</sub>O<sub>2</sub> in 0.887mL water

Revision as of 08:54, 10 October 2013

Protein Re-engineering Main project page
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Objective

Take kinetic data for Mb (lyophilized from KCl/acetate buffer) catalyzed o-phenylenediamine + H2O2 reaction in water (45C) and methanol (5, 15, 25C)

Procedure and Data

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 1.3mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
    2. OPD
      1. 3.5mg OPD in 3.5mL water
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL water
  2. Experimental Run
    1. Sample chamber set to 45C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Acetate_water_45_SCANNUMBER.txt
    6. 0.225mL Mb stock solution added just before the third scan
    7. This is denoted in the data file for the third scan



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