User:Matt Hartings/Notebook/Photosynthesis/2013/09/27: Difference between revisions

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## 0.227mL 30% H<sub>2</sub>O<sub>2</sub> in 1.773mL water
## 0.227mL 30% H<sub>2</sub>O<sub>2</sub> in 1.773mL water


<u>Samples 1</u>
<u>Sample 1</u>
# Sample chamber set to 25C and to stir
# Sample chamber set to 25C and to stir
# 2.025mL OPD
# 2.025mL OPD
Line 31: Line 31:
## Wait 1 minute after each scan
## Wait 1 minute after each scan
## Stop after 2 hours
## Stop after 2 hours
## Files saved as: Mb_OPD_H2O2_Citrate_H20_SCANNUMBER.txt
## Files saved as: Mb_OPD_H2O2_Citrate_H20_25_SCANNUMBER.txt
# 0.225mL Mb added just before the third scan
# 0.225mL Mb added just before the third scan
## This is denoted in the data file for the third scan
## This is denoted in the data file for the third scan


<u>Sample 2</u>
# Sample chamber set to 15C and to stir
# 2.025mL OPD *Note---> The OPD had already started to turn yellow in the container. Must make a fresh sample for each run. This is probably what went wrong with Dhea and Paul's stuff this summer. [[User:Matt Hartings|Matt Hartings]]
# 0.75mL H<sub>2</sub>O<sub>2</sub>
# Start Data Collection
## 1ms Integration
## 10 scan average
## Wait 1 minute after each scan
## Stop after 2 hours
## Files saved as: Mb_OPD_H2O2_Citrate_H20_15_SCANNUMBER.txt
# 0.225mL Mb added just before the third scan
## This is denoted in the data file for the third scan


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Revision as of 12:34, 27 September 2013

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Objective

Running Mb catalyzed H2O2 and o-phenylenediamine reaction today.

Procedure and Data

Stock solutions

  1. Myoglobin
    1. 2.0mg (Mb:KCl lyophilized from Citrate, 5mg:500mg) in 1.0mL water
  2. OPD
    1. 11.5mg OPD in 11.5mL water
  3. H2O2
    1. 0.227mL 30% H2O2 in 1.773mL water

Sample 1

  1. Sample chamber set to 25C and to stir
  2. 2.025mL OPD
  3. 0.75mL H2O2
  4. Start Data Collection
    1. 1ms Integration
    2. 10 scan average
    3. Wait 1 minute after each scan
    4. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_H20_25_SCANNUMBER.txt
  5. 0.225mL Mb added just before the third scan
    1. This is denoted in the data file for the third scan

Sample 2

  1. Sample chamber set to 15C and to stir
  2. 2.025mL OPD *Note---> The OPD had already started to turn yellow in the container. Must make a fresh sample for each run. This is probably what went wrong with Dhea and Paul's stuff this summer. Matt Hartings
  3. 0.75mL H2O2
  4. Start Data Collection
    1. 1ms Integration
    2. 10 scan average
    3. Wait 1 minute after each scan
    4. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_Citrate_H20_15_SCANNUMBER.txt
  5. 0.225mL Mb added just before the third scan
    1. This is denoted in the data file for the third scan


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