User:Matt Hartings/Notebook/Photosynthesis/2013/06/21: Difference between revisions
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== | ==POM Synthesis== | ||
Today I'm performing the synthesis of K<sub>8</sub>HP<sub>2</sub>W<sub>15</sub>V<sub>3</sub>O<sub>62</sub>-9H<sub>2</sub>O following [[Hartings_AU_Photosynthesis_Lab:Protocols/K8HP2W15V3O62-9H2O|this]] protocol. The only difference is that I"m scaling the synthesis down by a factor of 10. | |||
# 0.4018g of sodium metavanadate is disolved in 70mL of hot water and cooled to room temperature (very pale green solid ... makes a clear liquid) | |||
# 1.6mL of 6M HCl is added (solution turns a beautiful golden color) | |||
# 4.639g of [[User:Matt_Hartings/Notebook/Photosynthesis/2013/06/18|Na<sub>12</sub>P<sub>2</sub>W<sub>15</sub>O<sub>56</sub>]] is added slowly with vigorous stirring. (The solution tuned a deep red-orange color) | |||
# Solution was stirred for 10 minutes | |||
# 10.0369g of KCl was added to precipitate the POM | |||
# Solid was removed by filtration. (I let the vacuum filtration go for an extra hour to try and dry the solid. But the solid was still quite wet when I collected it. Mass = 6.8293g) | |||
# Solid was recrystallized from hot water (pH 1.5). (Started with around 15mL of water, removed from heat when crystals started appearing at around 7.5mL) | |||
# Placed in the refrigerator over the weekend. | |||
Revision as of 11:51, 21 June 2013
 Protein Re-engineering
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POM SynthesisToday I'm performing the synthesis of K8HP2W15V3O62-9H2O following this protocol. The only difference is that I"m scaling the synthesis down by a factor of 10.
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