User:Matt Hartings/Notebook/Photosynthesis/2013/04/02: Difference between revisions
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# 1.134 ml 30% H2O2 +8.866 ml Methanol | # 1.134 ml 30% H2O2 +8.866 ml Methanol | ||
# 8.9mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of methanol. | # 8.9mg of (5.7mg myoglobin +0.4861g KCl lyophilized from 20mL of 10mM citrate pH 3) in 1 mL of methanol. | ||
<u>Samples were made by</u> | |||
# 2.7 mL of the OPD solution | |||
# 0.3 mL of the Mb solution | |||
# Start the scan | |||
## 1,000 us integration time | |||
## Average over 500 scans | |||
## Run for 10 minutes (600,000,000 us) | |||
# Add 1mL of the H2O2 solution | |||
## Added after scan reached 1% | |||
# Kinetics scans were run at 5, 15, 25, 35, and 45C. Several before and after spectra were also taken, as were the dark and reference signal spectra. | |||
# Absorbance measurements were calculated using the following equation: A = -log[(SampleSignal-DarkSignal)/(ReferenceSignal-DarkSignal)] | |||
Shown below are kinetics traces and absorbance scans for the different temperatures | |||
Revision as of 07:28, 3 April 2013
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Mb Catalysis in MethanolRunning tests on myoglobin catalysis in methanol today. Catalysis is on the conversion of o-phenylenediamine (OPD) into 2,3-diaminophenazine (DAPN)
Stock solutions used
Samples were made by
Shown below are kinetics traces and absorbance scans for the different temperatures
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