User:Matt Hartings/Notebook/Photosynthesis/2013/03/18: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/03/18 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
 
No edit summary
Line 10: Line 10:




==Objective==
==Mb in DMSO==
Learn how to maintain an OpenWetWare Notebook.


Starting with the lyophilized samples that I made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/11|the 11th]] I am going to make samples in DMSO with crown or (nBu<sub>4</sub>)NPF<sub>6</sub> made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/12|the 12th]]
# Starting makeup of sample lyophilized at pH 3
### 0.0115g Mb
### 0.2005g D-sorbitol
### 0.110g (1450MW) PEG
### 10mL 10mM citrate pH 3
## (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
## (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
# Starting makeup of sample lyophilized at pH 5
### 0.0110g Mb
### 0.1946g D-sorbitol
### 0.100g (1450MW) PEG
### 10mL 10mM acetate pH 5
## (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
## (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
# Starting makeup of sample lyophilized at pH 7
### 0.020g Mb
### 0.1998g D-sorbitol
### 0.1085g (1450MW) PEG
### 10mL 10mM phosphate pH 7
## (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
## (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
# Starting makeup of sample lyophilized at pH 9
### 0.0118g Mb
### 0.1847g D-sorbitol
### 0.106g (1450MW) PEG
### 10mL 10mM tris pH 9
## (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
## (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 10:06, 18 March 2013

Protein Re-engineering <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>



Mb in DMSO

Starting with the lyophilized samples that I made on the 11th I am going to make samples in DMSO with crown or (nBu4)NPF6 made on the 12th

  1. Starting makeup of sample lyophilized at pH 3
      1. 0.0115g Mb
      2. 0.2005g D-sorbitol
      3. 0.110g (1450MW) PEG
      4. 10mL 10mM citrate pH 3
    2. (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
  2. Starting makeup of sample lyophilized at pH 5
      1. 0.0110g Mb
      2. 0.1946g D-sorbitol
      3. 0.100g (1450MW) PEG
      4. 10mL 10mM acetate pH 5
    2. (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
  3. Starting makeup of sample lyophilized at pH 7
      1. 0.020g Mb
      2. 0.1998g D-sorbitol
      3. 0.1085g (1450MW) PEG
      4. 10mL 10mM phosphate pH 7
    2. (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
  4. Starting makeup of sample lyophilized at pH 9
      1. 0.0118g Mb
      2. 0.1847g D-sorbitol
      3. 0.106g (1450MW) PEG
      4. 10mL 10mM tris pH 9
    2. (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Matt_Hartings/Notebook/Photosynthesis/2013/03/18&oldid=683699"