User:Matt Hartings/Notebook/Photosynthesis/2013/03/18

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(Autocreate 2013/03/18 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Current revision (13:06, 18 March 2013) (view source)
 
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==Objective==
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==Mb in DMSO==
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Learn how to maintain an OpenWetWare Notebook.
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Starting with the lyophilized samples that I made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/11|the 11th]] I am going to make samples in DMSO with crown or (nBu<sub>4</sub>)NPF<sub>6</sub> made on [[User:Matt_Hartings/Notebook/Photosynthesis/2013/03/12|the 12th]]
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# Starting makeup of sample lyophilized at pH 3
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### 0.0115g Mb
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### 0.2005g D-sorbitol
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### 0.110g (1450MW) PEG
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### 10mL 10mM citrate pH 3
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## (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
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## (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
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# Starting makeup of sample lyophilized at pH 5
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### 0.0110g Mb
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### 0.1946g D-sorbitol
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### 0.100g (1450MW) PEG
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### 10mL 10mM acetate pH 5
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## (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
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## (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
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# Starting makeup of sample lyophilized at pH 7
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### 0.020g Mb
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### 0.1998g D-sorbitol
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### 0.1085g (1450MW) PEG
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### 10mL 10mM phosphate pH 7
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## (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
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## (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
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# Starting makeup of sample lyophilized at pH 9
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### 0.0118g Mb
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### 0.1847g D-sorbitol
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### 0.106g (1450MW) PEG
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### 10mL 10mM tris pH 9
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## (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu<sub>4</sub>)NPF<sub>6</sub>
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## (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6
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Mb in DMSO

Starting with the lyophilized samples that I made on the 11th I am going to make samples in DMSO with crown or (nBu4)NPF6 made on the 12th

  1. Starting makeup of sample lyophilized at pH 3
      1. 0.0115g Mb
      2. 0.2005g D-sorbitol
      3. 0.110g (1450MW) PEG
      4. 10mL 10mM citrate pH 3
    2. (Sample 1) Measured 3.8mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 5.8mg of solid and added 1mL of DMSO with 18-crown-6
  2. Starting makeup of sample lyophilized at pH 5
      1. 0.0110g Mb
      2. 0.1946g D-sorbitol
      3. 0.100g (1450MW) PEG
      4. 10mL 10mM acetate pH 5
    2. (Sample 1) Measured 6.2mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 9.8 mg of solid and added 1mL of DMSO with 18-crown-6
  3. Starting makeup of sample lyophilized at pH 7
      1. 0.020g Mb
      2. 0.1998g D-sorbitol
      3. 0.1085g (1450MW) PEG
      4. 10mL 10mM phosphate pH 7
    2. (Sample 1) Measured 11.6 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 12.2 mg of solid and added 1mL of DMSO with 18-crown-6
  4. Starting makeup of sample lyophilized at pH 9
      1. 0.0118g Mb
      2. 0.1847g D-sorbitol
      3. 0.106g (1450MW) PEG
      4. 10mL 10mM tris pH 9
    2. (Sample 1) Measured 6.2 mg of solid and added 1mL of DMSO with (nBu4)NPF6
    3. (Sample 2) Measured 7.9 mg of solid and added 1mL of DMSO with 18-crown-6


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