User:Matt Hartings/Notebook/Photosynthesis/2013/03/12

From OpenWetWare

< User:Matt Hartings | Notebook | Photosynthesis | 2013 | 03(Difference between revisions)
Jump to: navigation, search
(Autocreate 2013/03/12 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Current revision (14:21, 12 March 2013) (view source)
 
Line 10: Line 10:
-
==Objective==
+
==Solvents for protein stabilization==
-
Learn how to maintain an OpenWetWare Notebook.
+
Prepping solvents for looking at protein stability in organic solvents.
 +
 
 +
# Acetonitrile
 +
## 0.4175g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL acetonitrile
 +
## 0.6752g 18-crown-6 + 25mL acetonitrile
 +
## 0.4033g (nBu)<sub>4</sub>NPF<sub>6</sub> +0.6756g 18-crown-6 + 25mL acetonitrile
 +
 
 +
# Hexanes
 +
## 0.4021g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL hexanes
 +
## 0.6697g 18-crown-6 + 25mL hexanes
 +
## 0.4012g (nBu)<sub>4</sub>NPF<sub>6</sub> +0.6892g 18-crown-6 + 25mL hexanes
 +
 
 +
# DMSO
 +
## 0.4129g (nBu)<sub>4</sub>NPF<sub>6</sub> + 25mL DMSO
 +
## 0.6872g 18-crown-6 + 25mL DMSO
 +
## ran out of (nBu)<sub>4</sub>NPF<sub>6</sub> for this joint solvent with + 18-crown-6

Current revision

Protein Re-engineering Main project page
Previous entry      Next entry



Solvents for protein stabilization

Prepping solvents for looking at protein stability in organic solvents.

  1. Acetonitrile
    1. 0.4175g (nBu)4NPF6 + 25mL acetonitrile
    2. 0.6752g 18-crown-6 + 25mL acetonitrile
    3. 0.4033g (nBu)4NPF6 +0.6756g 18-crown-6 + 25mL acetonitrile
  1. Hexanes
    1. 0.4021g (nBu)4NPF6 + 25mL hexanes
    2. 0.6697g 18-crown-6 + 25mL hexanes
    3. 0.4012g (nBu)4NPF6 +0.6892g 18-crown-6 + 25mL hexanes
  1. DMSO
    1. 0.4129g (nBu)4NPF6 + 25mL DMSO
    2. 0.6872g 18-crown-6 + 25mL DMSO
    3. ran out of (nBu)4NPF6 for this joint solvent with + 18-crown-6



Personal tools