User:Matt Hartings/Notebook/Photosynthesis/2013/03/11: Difference between revisions
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== | ==Lyophilizing Mb== | ||
Lyophilizing Mb samples for studies in organic solvents | |||
# pH 3 | |||
## 0.0115g Mb | |||
## 0.2005g D-sorbitol | |||
## 0.110g (1450MW) PEG | |||
# 10mL 10mM citrate pH 3 | |||
# pH 5 | |||
## 0.0110g Mb | |||
## 0.1946g D-sorbitol | |||
## 0.100g (1450MW) PEG | |||
# 10mL 10mM acetate pH 5 | |||
# pH 7 | |||
## 0.020g Mb | |||
## 0.1998g D-sorbitol | |||
## 0.1085g (1450MW) PEG | |||
# 10mL 10mM phosphate pH 7 | |||
# pH 9 | |||
## 0.0118g Mb | |||
## 0.1847g D-sorbitol | |||
## 0.106g (1450MW) PEG | |||
# 10mL 10mM tris pH 9 | |||
#Take these samples and freeze them in liquid nitrogen. | |||
## All samples are reddish brown except for the acetate, which has a greenish color. | |||
Revision as of 12:37, 12 March 2013
 Protein Re-engineering
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Lyophilizing MbLyophilizing Mb samples for studies in organic solvents
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