User:Matt Hartings/Notebook/Photosynthesis/2013/02/26: Difference between revisions
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== | ==Protein Dialysis== | ||
In order to prepare the Mn-Hb samples for purification, I am dialyzing the samples. | |||
# Make 4L of 10mM Tris 50mM NaCl, pH 8.5 | |||
## Measured 11.681 g NaCl | |||
## Measured 4.860 g Tris | |||
# I split the crude Mn-Hb solution over 2 dialysis tubes (snakeskin). | |||
# The dialysis tubes were places in the buffer and am storing them in the cold room overnight | |||
Revision as of 08:23, 26 February 2013
 Protein Re-engineering
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Protein DialysisIn order to prepare the Mn-Hb samples for purification, I am dialyzing the samples.
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