User:Matt Hartings/Notebook/Photosynthesis/2013/02/12
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| + | ==DNA Gel== | ||
| + | Maddie ran several PCR runs last week to try to mutate K31C Asc Hb to K31C T109C Asc Hb. | ||
| + | I made an agarose gel (1% - 0.25g agarose in 25mL TAE Buffer) | ||
| + | The lanes in the gel are | ||
| + | # Ladder | ||
| + | # WT | ||
| + | # F1 | ||
| + | # F2 | ||
| + | # F3 | ||
| + | # F4 | ||
| - | + | (each lane consists of 5uL DNA and 1uL loading buffer). | |
| - | + | ||
| + | ==Hb extraction== | ||
| + | |||
| + | Last week we expressed WT Asc Mn-Hb and Ni-Hb. I am going to extract the Mn-Hb today. | ||
| + | # Sonicate: 30 seconds on, 30 seconds off, 3x | ||
| + | # balance in oak ridge tubes | ||
| + | # centrifuge for 2 hours | ||
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Revision as of 13:52, 12 February 2013
 Protein Re-engineering
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DNA GelMaddie ran several PCR runs last week to try to mutate K31C Asc Hb to K31C T109C Asc Hb. I made an agarose gel (1% - 0.25g agarose in 25mL TAE Buffer) The lanes in the gel are
(each lane consists of 5uL DNA and 1uL loading buffer). Hb extractionLast week we expressed WT Asc Mn-Hb and Ni-Hb. I am going to extract the Mn-Hb today.
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