User:Matt Hartings/Notebook/Photosynthesis/2013/01/22: Difference between revisions
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== | ==MOF== | ||
Abigail and I are doing a new MOF synthesis today with [https://macrocyclics.com/shop/product_info.php?cPath=2&products_id=69&osCsid=f9126a59350aa546308b0a8c52d5e244 DO2A]. | |||
Plan: 50 mg of DO2A (MW = 424.2) + 59.0 mg of Zn(Acetate)2·4H2O (MW = 291.5) ratio of 7 Zn for each 3 DO2A. This is from the ratio's from the papers for the synthesis of MOF-5 (4Zns:3ligands) but each of our ligands needs an extra Zn for the middle of the macrocycle. | |||
Actual: | |||
51.3mg of DO2A and 59.8mg of Zn(Acetate)2·4H2O. Add 2mL of diethylformamide. Put in oven (85C) at 11:23 am. | |||
==Mini-preps== | |||
Elaine, Maddie, Nyousha and I did minipreps with pQE-80 (kan<sub>R</sub>) and WT swMyoglobin in pT7-7 (amp<sub>R</sub>) | |||
Each miniprep had 5mL of LB and 5uL of appropriate antibiotic. | |||
They were innoculated from a glycerol stock of appropriate vector in NovaBlue cells. | |||
==Lyophilizing== | |||
We set up 3 more of the equine blood myoglobin samples that Dhea and Paul made for lyophilizing. Going to let them run 24 hours. | |||
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Revision as of 13:29, 23 January 2013
 Protein Re-engineering
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MOFAbigail and I are doing a new MOF synthesis today with DO2A. Plan: 50 mg of DO2A (MW = 424.2) + 59.0 mg of Zn(Acetate)2·4H2O (MW = 291.5) ratio of 7 Zn for each 3 DO2A. This is from the ratio's from the papers for the synthesis of MOF-5 (4Zns:3ligands) but each of our ligands needs an extra Zn for the middle of the macrocycle. Actual: 51.3mg of DO2A and 59.8mg of Zn(Acetate)2·4H2O. Add 2mL of diethylformamide. Put in oven (85C) at 11:23 am. Mini-prepsElaine, Maddie, Nyousha and I did minipreps with pQE-80 (kanR) and WT swMyoglobin in pT7-7 (ampR) Each miniprep had 5mL of LB and 5uL of appropriate antibiotic. They were innoculated from a glycerol stock of appropriate vector in NovaBlue cells. LyophilizingWe set up 3 more of the equine blood myoglobin samples that Dhea and Paul made for lyophilizing. Going to let them run 24 hours. | |
