User:Matt Hartings/Notebook/Photosynthesis/2013/01/18

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(Autocreate 2013/01/18 Entry for User:Matt_Hartings/Notebook/Photosynthesis)
Current revision (14:32, 18 January 2013) (view source)
 
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==Objective==
==Objective==
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Learn how to maintain an OpenWetWare Notebook.
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Measure stock DNA solutions
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==Procedure==
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Dilute samples (2 uL of DNA, 198 uL of water)
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Measure absorbance.
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Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL
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When I could, I used data sheets from IDT for epsilon values of oligos
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==Data==
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[[Image:20130118_DNA_UVVis.png|400px]]
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Important notes:
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# I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low.
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# Asc Hb T109C f 136ug/mL (from assumption)
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# Asc Hb T109C r 272ug/mL (from assumption)
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# sw Mb M131T f 60.5 mmol/mL (from data sheet)
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# sw Mb M131T r 10.3 mmol/mL (from data sheet)
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# sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet)
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# sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet)
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Objective

Measure stock DNA solutions

Procedure

Dilute samples (2 uL of DNA, 198 uL of water)

Measure absorbance.

Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL When I could, I used data sheets from IDT for epsilon values of oligos

Data

Important notes:

  1. I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low.
  2. Asc Hb T109C f 136ug/mL (from assumption)
  3. Asc Hb T109C r 272ug/mL (from assumption)
  4. sw Mb M131T f 60.5 mmol/mL (from data sheet)
  5. sw Mb M131T r 10.3 mmol/mL (from data sheet)
  6. sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet)
  7. sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet)


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