User:Matt Hartings/Notebook/Photosynthesis/2013/01/18
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==Objective== | ==Objective== | ||
| - | + | Measure stock DNA solutions | |
| + | ==Procedure== | ||
| + | Dilute samples (2 uL of DNA, 198 uL of water) | ||
| + | |||
| + | Measure absorbance. | ||
| + | |||
| + | Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL | ||
| + | When I could, I used data sheets from IDT for epsilon values of oligos | ||
| + | |||
| + | ==Data== | ||
| + | [[Image:20130118_DNA_UVVis.png|400px]] | ||
| + | |||
| + | Important notes: | ||
| + | # I need to do new mini/midi-preps for WT sw Mb and pQE-80 as the concentrations were just so low. | ||
| + | # Asc Hb T109C f 136ug/mL (from assumption) | ||
| + | # Asc Hb T109C r 272ug/mL (from assumption) | ||
| + | # sw Mb M131T f 60.5 mmol/mL (from data sheet) | ||
| + | # sw Mb M131T r 10.3 mmol/mL (from data sheet) | ||
| + | # sw Mb pQE80 clone f 95.1 mmol/mL (from data sheet) | ||
| + | # sw Mb pQE80 clone r 30.4 mmol/mL (from data sheet) | ||
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ObjectiveMeasure stock DNA solutions ProcedureDilute samples (2 uL of DNA, 198 uL of water) Measure absorbance. Assume: for dsDNA, an absorbance of 1 corresponds to a concentration of 50ug/mL and for oligos an absorbance of 1 corresponds to a concentration of 20ug/mL When I could, I used data sheets from IDT for epsilon values of oligos Data
Important notes:
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