User:Matt Hartings/Notebook/Photosynthesis/2013/01/04: Difference between revisions
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==Objective== | ==Objective== | ||
Prepping some work for the weeks ahead | Prepping some work for the weeks ahead |
Revision as of 09:19, 4 January 2013
Protein Re-engineering | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
ObjectivePrepping some work for the weeks ahead CloningPCR
Purifying PCR product
Restriction of PCR productIf you had a bright DNA band after PCR, use 20μL of the product. If you had a faint band, lyophilize the 50μL from the previous step and add 20μL water. The restriction enzymes that I need for pQE-80 are: BamHI and HindIII
Restriction of vectorRun the cuts at the end of the day
LigationThis also runs overnight
TransformProtocol uses electrocompetent cells. May have to see about using these |