User:Matt Hartings/Notebook/Photosynthesis/2012/09/13
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Current revision (13:04, 17 September 2012) (view source) (→Objective) |
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Extract Hb protein from cells. | Extract Hb protein from cells. | ||
| + | ==Procedure== | ||
| + | #Thaw cell pellets | ||
| + | #Run 3 cycles of ultrasonication | ||
| + | ##30seconds sonicating | ||
| + | ##30 seconds on ice | ||
| + | #Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water | ||
| + | #centrifuge at 18000rpm at 4C for 2 hours | ||
| + | #decant liquid from tubes into fresh falcon tubes. | ||
| + | #store in refrigerator | ||
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Current revision
 Protein Re-engineering
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ObjectiveExtract Hb protein from cells. Procedure
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