User:Matt Hartings/Notebook/Photosynthesis/2012/09/13

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(Objective)
Current revision (12:04, 17 September 2012) (view source)
(Objective)
 
Line 13: Line 13:
Extract Hb protein from cells.
Extract Hb protein from cells.
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==Procedure==
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#Thaw cell pellets
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#Run 3 cycles of ultrasonication
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##30seconds sonicating
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##30 seconds on ice
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#Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water
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#centrifuge at 18000rpm at 4C for 2 hours
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#decant liquid from tubes into fresh falcon tubes.
 +
#store in refrigerator
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Objective

Extract Hb protein from cells.

Procedure

  1. Thaw cell pellets
  2. Run 3 cycles of ultrasonication
    1. 30seconds sonicating
    2. 30 seconds on ice
  3. Balance centrifuge tubes (oak ridge tubes) containing sonicated cells with water
  4. centrifuge at 18000rpm at 4C for 2 hours
  5. decant liquid from tubes into fresh falcon tubes.
  6. store in refrigerator


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