User:Matt Hartings/Notebook/Photosynthesis/2012/09/11: Difference between revisions

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==Objective==
==Objective==
Learn how to maintain an OpenWetWare Notebook.
Prep for hemoglobin expression tomorrow
 
==Procedure==
Prepare autoclaved LB broth
#Place 25 g of LB in Fernbach flask
#Add 1 L of water
#Repeat to make a total of 4 flasks
#Place 0.875g of LB in 250mL ehrlenmeyer flask
#Add 35mL of water
#Repeat to make a total of 4 flasks
#Autoclave flasks
##2 runs each containing 2 fernbachs and 2ehrlenmeyers
 
Inoculate starter cultures
#Add 35uL of 100mg/mL ampicilin to each ehrlenmeyer
#Transfer culture from frozen glycerol stock of WT Ascaris Hb to starter culture flasks
#Shake overnight at 37C




Revision as of 08:22, 12 September 2012

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Objective

Prep for hemoglobin expression tomorrow

Procedure

Prepare autoclaved LB broth

  1. Place 25 g of LB in Fernbach flask
  2. Add 1 L of water
  3. Repeat to make a total of 4 flasks
  4. Place 0.875g of LB in 250mL ehrlenmeyer flask
  5. Add 35mL of water
  6. Repeat to make a total of 4 flasks
  7. Autoclave flasks
    1. 2 runs each containing 2 fernbachs and 2ehrlenmeyers

Inoculate starter cultures

  1. Add 35uL of 100mg/mL ampicilin to each ehrlenmeyer
  2. Transfer culture from frozen glycerol stock of WT Ascaris Hb to starter culture flasks
  3. Shake overnight at 37C



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