User:Matt Hartings/Notebook/Photosynthesis/2012/07/11: Difference between revisions
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#Place in PCR machine | #Place in PCR machine | ||
#Run DEL program. (Tamra had edited an older program) | #Run DEL program. (Tamra had edited an older program) | ||
## Start with 30 seconds at 98°C on the thermocycler. | |||
## Cycle through the following 40 times: | |||
##* 10 seconds at 98°C | |||
##* 30 seconds at 62°C | |||
##* 30 seconds at 72°C | |||
## A final extension step of 5 min was done at 72°C. | |||
## The thermocycler was then held at 4°C. | |||
Most components for this PCR came from the [http://www.neb.com/nebecomm/ManualFiles/manualE0553.pdf Phusion® High-Fidelity PCR Kit]. | |||
Revision as of 08:30, 11 July 2012
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ObjectiveStart cloning sperm whale myoglobin gene into pQE-80 vector PCR Cloning
Most components for this PCR came from the Phusion® High-Fidelity PCR Kit.
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