User:Matt Hartings/Notebook/Photosynthesis/2012/06/28

From OpenWetWare

< User:Matt Hartings | Notebook | Photosynthesis | 2012 | 06(Difference between revisions)
Jump to: navigation, search
(Objective)
Current revision (15:18, 30 June 2012) (view source)
(Objective)
 
Line 11: Line 11:
==Objective==
==Objective==
-
Prepare media for hemoglobin expression.
+
Prepare media for hemoglobin expression and set up starter cultures.
 +
==Procedure==
Starter cultures (x4)
Starter cultures (x4)
# 0.875g LB
# 0.875g LB
Line 22: Line 23:
# 1L water
# 1L water
# autoclave
# autoclave
 +
 +
Starter cultures (x4)
 +
# 35uL of 100mg/mL ampicilin was added to each starter culture flask
 +
# Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock
 +
# Flasks were placed in the shaker/incubator overnight (37C, 250rpm)

Current revision

Biomaterials Design Lab Main project page
Previous entry      Next entry



Objective

Prepare media for hemoglobin expression and set up starter cultures.

Procedure

Starter cultures (x4)

  1. 0.875g LB
  2. 35mL water
  3. autoclave

Expression cultures (x4)

  1. 25g LB
  2. 1L water
  3. autoclave

Starter cultures (x4)

  1. 35uL of 100mg/mL ampicilin was added to each starter culture flask
  2. Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock
  3. Flasks were placed in the shaker/incubator overnight (37C, 250rpm)



Personal tools