User:Matt Hartings/Notebook/Photosynthesis/2012/06/28
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< User:Matt Hartings | Notebook | Photosynthesis | 2012 | 06(Difference between revisions)
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Current revision (15:18, 30 June 2012) (view source) (→Objective) |
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==Objective== | ==Objective== | ||
| - | Prepare media for hemoglobin expression. | + | Prepare media for hemoglobin expression and set up starter cultures. |
| + | ==Procedure== | ||
Starter cultures (x4) | Starter cultures (x4) | ||
# 0.875g LB | # 0.875g LB | ||
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# 1L water | # 1L water | ||
# autoclave | # autoclave | ||
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| + | Starter cultures (x4) | ||
| + | # 35uL of 100mg/mL ampicilin was added to each starter culture flask | ||
| + | # Starter culture was inoculated with cells from the BL21(DE3)/pET-3d/WT Ascaris hemoglobin glycerol stock | ||
| + | # Flasks were placed in the shaker/incubator overnight (37C, 250rpm) | ||
Current revision
 Biomaterials Design Lab
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ObjectivePrepare media for hemoglobin expression and set up starter cultures. ProcedureStarter cultures (x4)
Expression cultures (x4)
Starter cultures (x4)
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