User:Matt Hartings/Notebook/Photosynthesis/2012/06/25: Difference between revisions
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==Description== | ==Description== | ||
# | # Thaw resuspended cells | ||
# Sonicate at power level 12 on Misonix XL-2000. Sonicate for 30 seconds. Rest on ice for 30 seconds. | |||
# Place in centrifuge tubes and balance weight to near 0.01g | |||
# Centrifuge for 2 hours at 18000rpm and 4C | |||
# Dialyze into 25mM Tris, 50mM NaCl pH 8.3 overnight in cold room. | |||
==Data== | ==Data== |
Revision as of 11:59, 27 June 2012
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ObjectiveExtract hemoglobin from Ecoli previously used in expression. Description
Data
NotesThis area is for any observations or conclusions that you would like to note.
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