User:Matt Hartings/Notebook/Photosynthesis/2012/06/13: Difference between revisions
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==Description== | ==Description== | ||
1L of 25mM Tris, 50mM NaCl pH8 | |||
3.029g Tris, 2.922g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter | |||
1L of 25mM Tris, 1M NaCl pH8 | |||
3.029g Tris, 58.44g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter | |||
==Data== | ==Data== |
Revision as of 11:14, 13 June 2012
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ObjectiveMaking FPLC buffers Description1L of 25mM Tris, 50mM NaCl pH8 3.029g Tris, 2.922g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter 1L of 25mM Tris, 1M NaCl pH8 3.029g Tris, 58.44g NaCl, Add 900mL of water, Adjust pH with HCl, Add water up to 1L, Filter Data
NotesThis area is for any observations or conclusions that you would like to note.
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