User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/29: Difference between revisions

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# Lysozyme, pH 5, 80C
# Lysozyme, pH 5, 80C


==Description==
==BSA AuNP Fluorescence==
# Add experimental record here. Include what, how, and why...
<u>3 mL Sample</u>
# 0.25 mM AuCl3
## Stock concentration 4.27 mM
## Volume Added: 176 μL
# 3.125 μM BSA
## Stock Concentration 52.6 μM
## Volume Added: 178 μL
# pH 6
## Stock Concentration 1 mM HCl
## Volume Added: 3 μL
# Volume water added: 2643 μL
 
<u>UV-Vis of sample</u>
 
[[Image:20160929 mrh BSA AuNP UVVis.png]]
 
<u>Fluorescence scans</u>
 
# Acquisition information
## Excitation: 290 nm, 10 nm slit width
## Emission: 310-540 nm, 10 nm slit width
## 100 nm/minute
## 61 measurements, 180 second delay between measurements
 
[[Image:20190929 mrh BSAAuNP IntegratedI.png]]
 
[[Image:20190929 mrh BSAAuNP EmissionMax.png]]
 
[[Image:20190929 mrh BSAAuNP scans1.png]]
 
[[Image:20190929 mrh BSAAuNP scans2.png]]
 
[[Image:20190929 mrh BSAAuNP scans3.png]]
 
 
==Lysozyme Fluorescence==
<u>3 mL Sample</u>
# 0.5 μM Lysozyme
## Stock Concentration 210 μM
## Volume Added: 7.1 μL
# pH 5
## Stock Concentration 1 mM HCl
## Volume Added: 30 μL
# Volume Water Added: 2963 μL
 
<u>UV-Vis of sample</u>
 
[[Image:20160929 mrh Lysozyme pH5 UVVis.png]]
 
<u>Fluorescence scans</u>
 
# Acquisition information
## Excitation: 290 nm, 10 nm slit width
## Emission: 310-540 nm, 10 nm slit width
## 100 nm/minute
## 61 measurements, 180 second delay between measurements
 
[[Image:20160929 mrh LysozymepH5 IntegratedI.png]]
 
[[Image:20160929 mrh LysozymepH5 EmissionMax.png]]
 
[[Image:20160929 mrh LysozymepH5 SelectedScans.png]]


==Data==
==Data==

Revision as of 18:58, 29 September 2016

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Objective

Run fluorescence scans for

  1. BSA, AuNP, pH 6, 80C
  2. Lysozyme, pH 5, 80C

BSA AuNP Fluorescence

3 mL Sample

  1. 0.25 mM AuCl3
    1. Stock concentration 4.27 mM
    2. Volume Added: 176 μL
  2. 3.125 μM BSA
    1. Stock Concentration 52.6 μM
    2. Volume Added: 178 μL
  3. pH 6
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 3 μL
  4. Volume water added: 2643 μL

UV-Vis of sample

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements


Lysozyme Fluorescence

3 mL Sample

  1. 0.5 μM Lysozyme
    1. Stock Concentration 210 μM
    2. Volume Added: 7.1 μL
  2. pH 5
    1. Stock Concentration 1 mM HCl
    2. Volume Added: 30 μL
  3. Volume Water Added: 2963 μL

UV-Vis of sample

Fluorescence scans

  1. Acquisition information
    1. Excitation: 290 nm, 10 nm slit width
    2. Emission: 310-540 nm, 10 nm slit width
    3. 100 nm/minute
    4. 61 measurements, 180 second delay between measurements

Data

  • Add data and results here...

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.




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