User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/26: Difference between revisions
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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | |style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==Data== | ==Data== | ||
<u>Stock solutions</u> | |||
# Lysine | |||
## 3.93 mg lysine | |||
## to 10.0 mL water | |||
## 182.7 g/mol | |||
## 2.15 mM | |||
# AuCl3 | |||
## 32.39 mg AuCl3 | |||
## to 25.0 mL water | |||
## 303.3 g/mol | |||
## 4.27 mM | |||
<u>Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4</u> | |||
# 300μL 1 mM HCl | |||
# 176 μL AuCl3 | |||
# 192 μL lysine | |||
# 2332 μL water | |||
<u>UV-Vis Spectra</u> | |||
I took UV-Vis spectra before and after the fluorescence scans. | |||
[[Image:20160926 mrh LysineAuCl3pH480C UVVis1.png]] | |||
[[Image:20160926 mrh LysineAuCl3pH480C UVVis2.png]] | |||
<u>Picture of reaction product</u> | |||
[[Image:20160926 mrh lysaucl3 result.JPG|400px]] | |||
<u>Fluorescence Spectra</u> | |||
# 290 nm excitation, 10.0 nm slit width | |||
# Emission: 310-540 nm, 10 nm slit width | |||
# 100 nm/min | |||
[[Image:20160926 mrh lysaucl3 IntegratedI.png]] | |||
[[Image:20160926 mrh lysaucl3 EmissionMax.png]] | |||
[[Image:20160926 mrh lysaucl3 scans1.png]] | |||
[[Image:20160926 mrh lysaucl3 scans2.png]] | |||
==Notes== | ==Notes== |
Latest revision as of 02:02, 27 September 2017
Biomaterials Design Lab | Main project page Previous entry Next entry |
ObjectiveRunning a fluorescence scan of AuCl3+lysine Description
DataStock solutions
UV-Vis Spectra I took UV-Vis spectra before and after the fluorescence scans. Picture of reaction product Fluorescence Spectra
NotesThis area is for any observations or conclusions that you would like to note.
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