User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/26: Difference between revisions

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|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #EEE"|[[Image:BDLlogo_notext_lr.png|128px]]<span style="font-size:22px;"> Biomaterials Design Lab</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Data==
==Data==
* Add data and results here...
<u>Stock solutions</u>
# Lysine
## 3.93 mg lysine
## to 10.0 mL water
## 182.7 g/mol
## 2.15 mM
# AuCl3
## 32.39 mg AuCl3
## to 25.0 mL water
## 303.3 g/mol
## 4.27 mM
 
 
<u>Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4</u>
# 300μL 1 mM HCl
# 176 μL AuCl3
# 192 μL lysine
# 2332 μL water
 
<u>UV-Vis Spectra</u>
 
I took UV-Vis spectra before and after the fluorescence scans.
 
[[Image:20160926 mrh LysineAuCl3pH480C UVVis1.png]]
 
[[Image:20160926 mrh LysineAuCl3pH480C UVVis2.png]]
 
<u>Picture of reaction product</u>
 
[[Image:20160926 mrh lysaucl3 result.JPG|400px]]
 
<u>Fluorescence Spectra</u>
# 290 nm excitation, 10.0 nm slit width
# Emission: 310-540 nm, 10 nm slit width
# 100 nm/min
 
[[Image:20160926 mrh lysaucl3 IntegratedI.png]]
 
[[Image:20160926 mrh lysaucl3 EmissionMax.png]]
 
[[Image:20160926 mrh lysaucl3 scans1.png]]
 
[[Image:20160926 mrh lysaucl3 scans2.png]]


==Notes==
==Notes==

Latest revision as of 02:02, 27 September 2017

Biomaterials Design Lab Main project page
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Objective

Running a fluorescence scan of AuCl3+lysine

Description

  1. Add experimental record here. Include what, how, and why...

Data

Stock solutions

  1. Lysine
    1. 3.93 mg lysine
    2. to 10.0 mL water
    3. 182.7 g/mol
    4. 2.15 mM
  2. AuCl3
    1. 32.39 mg AuCl3
    2. to 25.0 mL water
    3. 303.3 g/mol
    4. 4.27 mM


Sample: 0.25mM AuCl3, 137.5 μM Lysine (chosen for rough concentration of lysine in lysozyme AuNP samples), pH 4

  1. 300μL 1 mM HCl
  2. 176 μL AuCl3
  3. 192 μL lysine
  4. 2332 μL water

UV-Vis Spectra

I took UV-Vis spectra before and after the fluorescence scans.

Picture of reaction product

Fluorescence Spectra

  1. 290 nm excitation, 10.0 nm slit width
  2. Emission: 310-540 nm, 10 nm slit width
  3. 100 nm/min

Notes

This area is for any observations or conclusions that you would like to note.


Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.